基于转录组测序的不同光学分光膜下生菜叶片的基因表达差异分析  

Analysis of Differentially Expressed Genes in Lettuce Leaves Under Different Optical Spectrophotometric Films Based on Transcriptome Sequencing

作　　者：周念念 高洁 丁明珠 安玉兰 翟克清 史加银 甘德芳[1] 刘文[3] ZHOU Niannian;GAO Jie;DING Mingzhu;AN Yulan;ZHAI Keqing;SHI Jiayin;GAN Defang*;LIU Wen(School of Horticulture,Anhui Agricultural University,Hefei,Anhui 230036,China;Agricultural Station of Jingong Town,Huaining,Anqing,Anhui 246100,China;School of Physical Sciences,University of Science and Technology of China,Hefei,Anhui 230026,China)

机构地区：[1]安徽农业大学园艺学院,安徽合肥230036 [2]怀宁县金拱镇农业站,安徽安庆246100 [3]中国科学技术大学物理学院,安徽合肥230026

出　　处：《热带作物学报》2020年第2期323-332,共10页Chinese Journal of Tropical Crops

基　　金：安徽省高等学校自然科学研究重点项目(No.KJ2019A0188);研究生创新基金(No.2019ysj-9);大学生创新项目(No.201810364177)。

摘　　要：新型光伏农业是在满足农作物生长的光照需求下进行光伏发电。为研究不同光学分光膜下生菜叶片的差异基因表达情况,以‘阿迪娜’生菜为试验材料,利用转录组测序(RNA-seq)技术分析在覆盖红蓝滤光膜(RBFF)和远红光截止膜(RICF)条件下生菜叶片的基因表达水平。结果表明,RBFF条件下有2685个基因存在差异表达,其中有1380个上调表达基因,1305个下调表达基因。RICF条件下有3264个基因差异表达,其中上调表达基因有881个,下调表达基因2383个。进一步分析发现,1498个差异表达基因在RBFF和RICF条件下均表达,1187个基因在RBFF条件下差异表达,1766个在RICF条件下差异表达。从上述差异表达基因中筛选出10个可能与光胁迫相关的基因,实时荧光定量验证转录组数据的可靠性。结果表明,大部分基因的表达情况与测序结果一致,说明转录组数据是可靠的。光响应相关基因的表达分析结果显示,RBFF覆盖下大部分基因在覆盖15 d时大量表达,达到表达高峰。RICF下的3个基因表达量较高,其余的表达量较低,大部分基因在15~21 d时达到表达高峰。本研究为进一步挖掘生菜基因组中光响应基因以及揭示生菜对光响应的分子机制提供理论依据和数据支持。New photovoltaic agriculture can generate photovoltaic power under the light demand of crop growth.To study the differential gene expression in lettuce leaves under different spectrophotometric films,the‘Adina’lettuce variety was used as the experimental material,and the gene expression level of lettuce leaves under the conditions of red and blue filter film(RBFF)and far infrared cut-off film(RICF)was analyzed by the transcriptome sequencing(RNA-seq)technique.The results showed that 2685 genes were differentially expressed in RBFF,including 1380 up-regulated genes and 1305 down-regulated genes;3264 genes were differentially expressed in RICF,of which 881 were up-regulation and 2383 down-regulation.Further analysis revealed that 1498 differentially expressed genes were expressed in both RBFF and RICF,1187 genes were differentially expressed in the RBFF,while 1766 were differentially expressed in RICF.Ten genes related to light stress were screened from the differentially expressed genes,and the reliability of transcriptome data was verified by qRT-PCR.The results of expression analysis of light response related genes showed that most of the genes under red-blue filter film were expressed in large quantities,and the expression reached peak at 15 d;under far infrared cut-off film,the expression of three genes was higher,while the expression of others was lower.The expression of most genes reached peak at 15-21 d.This study would provide a theoretical basis and data support for further exploring photoresponsive genes in the lettuce genome and revealing the molecular mechanism of the lettuce response to light.

关 键 词：生菜 光学分光膜 转录组分析 实时荧光定量PCR(qRT-PCR) 

分 类 号：Q949.783.5[生物学—植物学] Q786