花叶芦竹组织培养快繁技术研究  

Study on the technique of rapid propagation in tissue culture of Arundo donax var. versicolor

作　　者：严员英[1] 周莉荫[1] 朱仔伟[1] 任琼[1] 万方 Yan Yuanying;Zhou Liyin;Zhu Ziwei;Ren Qiong;Wan Fang(Jiangxi Academy of Forestry,Nanchang Jiangxi 330013,China)

机构地区：[1]江西省林业科学院

出　　处：《南方林业科学》2020年第1期17-20,共4页South China Forestry Science

基　　金：2017年江西省林业科学院公益项目“鄱阳湖滩涂地长苞香蒲花叶芦竹高效生态经营模式研究”

摘　　要：以花叶芦竹为试材,研究外植体不同类型、处理方式和启动培养基生长激素组合。结果表明:采用花叶芦竹新生侧枝茎段(带芽)作为外植体灭菌效果最好,根部和茎杆侧芽作为外植体染菌率高,不利于无菌体系的建立;接种前对芽点进行剥除苞片处理有利于缩短无菌体系建立的周期;适宜的启动培养基为MS+4.0 mol·L^-1 6-BA+2.0 mol·L^-1 IAA;最佳增殖培养基为MS+4.0 mol·L^-1 6-BA+1.6 mol·L^-1 IAA,增殖系数6.2。Arundo donax var.versicolor were taken as the experimental material in this study.Factors affecting establishment of sterility system in vitro of A.donax var.versicolor which were studies includes sources of explants,treatments,and basal medium.The results showed that lateral stem with buds were beneficial to the establishment of sterility system.Before inoculation,the treatment of bud point was helpful to shorten the period of the establishment of sterility system.The appropriate starter culture medium is MS+4.0 mol·L^-1 6-BA+2.0 mol·L^-1 IAA.The best medium of proliferation was MS+4.0 mol·L^-1 6-BA+1.6 mol·L^-1 IAA,multiplication times was 6.2.

关 键 词：短序润楠 AM菌 生长 光合作用 

分 类 号：S795.8[农业科学—林木遗传育种] S723.1326[农业科学—林学]