普通油茶INDEHISCENT同源基因的鉴别  

Identification of a homologue to the Arabidopsis thaliana INDEHISCENT gene in Camellia oleifera

作　　者：赵松子[1] Zhao Songzi(Jiangxi Camellia oleifera Germplasm Resources Protection and Utilization Laboratory,Jiangxi Academy of Forestry,Nanchang Jiangxi 330013,China)

机构地区：[1]江西省林业科学院·江西省油茶种质资源保护与利用实验室

出　　处：《南方林业科学》2020年第1期32-34,共3页South China Forestry Science

基　　金：江西省科技厅科技计划项目“油茶超级育种与配套栽培关键技术研究”(项目编号：20143ACF60013)

摘　　要：拟南芥(Arabidopsis thaliana)INDEHISCENT基因(At4g00120)是一个参与果荚开裂的基因。以普通油茶(Camellia oleifera)花蕾为材料进行转录组分析,得到一条INDEHISCENT基因同源片段;从普通油茶基因组三代测序数据中鉴别出一条含有该片段的基因组DNA序列;最后,得到油茶INDEHISCENT同源基因全长CDS序列,命名为CoIND。用拟南芥bHLH基因家族的18条蛋白质及CoIND的保守序列构建进化树,结果表明,CoIND与At4g00120在一个分支,表明其为At4g00120的同源基因。CoIND基因的的鉴别,为培育蒴果不裂果、适应机械化采收的油茶新品种奠定了基础。In Arabidopsis thaliana,INDEHISCENT gene(At4g00120)is the primary factor required for silique shattering.Transcriptome analysis was conducted with Camellia oleifera flower buds.A partial CDS homologous to At4g00120 was obtained in C.oleifera.Then,a long DNA fragment containing the CDS was identified from the whole genome SMRT sequence data.Finally,a full-length CDS was identified and named CoIND.bHLHs phylogenetic tree analysis revealed that CoIND was a homologue to the Arabidopsis INDEHISCENT.Identification of CoIND may help to creat new C.oleifera varieties with indehiscent capsules and adaptation for harvesting machinery.

关 键 词：普通油茶 拟南芥 不裂果 同源基因 蒴果 

分 类 号：S794.4[农业科学—林木遗传育种] Q343.17[农业科学—林学]