金荞麦对脂多糖诱导小鼠小肠炎症的保护作用  

Protective Effects of Fagopyrum dibotrys on Lipopolysaccharide-induced Intestinal Inflammation in Mice

作　　者：谭娅 甘麦邻[2] 范源 王婧[1] 张雄 黄波 朱砺 尚以顺[3] 史开志 TAN Ya;GAN Mailin;FAN Yuan;WANG Jing;ZHANG Xiong;HUANG Bo;ZHU Li;SHANG Yishun;SHI Kaizhi(Institute of Animal Husbandry and Veterinary,Guizhou Academy of Agricultural Science,Guiyang 550005,China;College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China;Guizhou Institute of Prataculture,Guizhou Academy of Agricultural Science,Guiyang 550005,China)

机构地区：[1]贵州省农业科学院畜牧兽医研究所,贵阳550005 [2]四川农业大学动物科技学院,成都611130 [3]贵州省农业科学院草业研究所,贵阳550005

出　　处：《中国畜牧兽医》2020年第2期597-604,共8页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省农科院专项(黔农科院专项[2016]031号);贵州省科技厅农业科技支撑计划(黔科合支撑[2016]2509号,黔科合支撑[2018]2280号);贵州省科技厅生猪健康养殖服务企业行动计划(黔科合服企[2015]4003号);贵州省现代生猪产业技术体系建设(GZCYTX201909)

摘　　要：为探究金荞麦对脂多糖(LPS)诱导的小鼠小肠炎症的保护作用,试验使用雌性ICR小鼠,前期14 d饮水添加不同浓度金荞麦和阳性对照品——芦丁(Rutin),随后腹腔注射LPS(10 mg/kg)构建小肠急性炎症模型,通过观察小鼠行为变化、血常规检测、组织切片和炎症相关基因定量检测等评估效果。结果发现,腹腔注射10 mg/kg的LPS可成功诱导小鼠急性肠炎;添加金荞麦能够剂量依赖性地降低LPS诱导的肠炎小鼠体重损失。血常规检测显示,腹腔注射LPS后导致小鼠血液白细胞数(WBC)、红细胞数(RBC)、血红蛋白(HGB)、血小板数目(PLT)显著下降,添加金荞麦或芦丁能一定程度缓解由于LPS引起的血常规异常。小肠切片HE染色结果显示,腹腔注射LPS后小肠固有层中间出现大量灶性的炎性细胞浸润,腺上皮可见少量的炎性细胞浸润,肠道细胞排列紊乱,可见核分裂及核碎裂,同时黏膜充血、出血严重;添加金荞麦或芦丁能一定程度抑制炎性细胞浸润和黏膜出血。实时荧光定量PCR结果显示,腹腔注射LPS能显著促进小肠促炎性因子IL-6、TNF-α和IL-1β的表达,添加金荞麦能剂量依赖性下调TNF-α和IL-1β的表达水平。以上结果表明饮水添加金荞麦和芦丁可以有效缓解LPS诱导的小鼠小肠炎症,金荞麦浓度为12%的添加剂量在部分指标上可达到或接近灌胃100 mg/kg芦丁的效果。To explore the protective effect of Fagopyrum dibotrys on lipopolysaccharide(LPS)-induced inflammation model of the small intestine in mice,ICR mice were selected in the experiment.Different concentration of Fagopyrum dibotrys and the positive control-Rutin were added in drinking water for 14 days treatment,then intraperitoneal injection of LPS(10 mg/kg)was used to construct the model of acute enteritis.Finally,the results were evaluated by behavior changes,routine blood tests,histological sections and gene expressions of inflammation-related genes.The results showed that the intraperitoneal injection of 10 mg/kg LPS could successfully induced acute enteritis in mice.The addition of Fagopyrum dibotrys could reduce the weight loss of LPS-induced acute enteritis in a dose-dependent manner.Blood routine test showed that intraperitoneal injection of LPS caused a significant decrease in white blood count(WBC),red blood count(RBC),hemoglobin(HGB)and platelet count(PLT)in mice,and the addition of Fagopyrum dibotrys or Rutin could alleviate the blood routine abnormality caused by LPS injection to a certain extent.The hematoxylin-eosin(HE)staining showed that after intraperitoneal injection of LPS,the focal inflammatory cells were found infiltrated into the lamina propria with a large number,as well as the glandular epithelium with a small number.Also,the disorganized intestinal cells,nuclear division and nuclear fragmentation were observed.Furthermore,severely mucosal hyperemia and hemorrhage were observed.To a certain extent,the infiltration of inflammatory cells and mucosa bleeding could be suppressed by adding Fagopyrum dibotrys or Rutin in mice.RT-PCR results showed that intraperitoneal injection of LPS could significantly promote the mRNA expression level of inflammatory factors(i.e.IL-6,TNF-αand IL-1β),and the expression of TNF-αand IL-1βdecreased by adding Fagopyrum dibotrys in a dose-dependent manner.The above results showed that Fagopyrum dibotrys or Rutin could effectively alleviate the inflammation of mice

关 键 词：金荞麦 脂多糖 肠炎 保护作用 

分 类 号：S816.5[农业科学—饲料科学]