虎眼万年青总皂苷诱导人乳腺癌MCF-7细胞凋亡机制研究  

Study on the apoptotic mechanisms of human breast cancer MCF-7 cells induced by total saponins of Ornithogalum caudatum ait.

作　　者：邹翔 周林 张月 宫甜 汲晨锋 曲中原[1] 刘学 李文兰[1] ZOU Xiang;ZHOU Lin;ZHANG Yue;GONG Tian;JI Chen-feng;QU Zhong-yuan;LIU Xue;LI Wen-lan(College of Pharmacy,Harbin University of Commerce Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学药学院抗肿瘤天然药物教育部工程研究中心

出　　处：《天然产物研究与开发》2020年第1期32-39,共8页Natural Product Research and Development

基　　金：国家自然科学基金青年基金(81102858);黑龙江省博士后科研启动金资助项目(LBH-Q16130);哈尔滨商业大学一般项目(17XN062)

摘　　要：为探讨虎眼万年青总皂苷(OCA-TS)诱导人乳腺癌MCF-7细胞凋亡的分子机制,本研究采用溴化四氮唑蓝(MTT)法检测OCA-TS对MCF-7细胞增殖的抑制作用,并观察细胞凋亡形态、检测细胞膜电位及凋亡相关蛋白含量变化。实验表明,OCA-TS可显著抑制人乳腺癌MCF-7细胞的增殖(IC 50为93.17μg/mL),电镜下细胞呈现典型的凋亡形态;流式细胞仪检测结果显示不同浓度的OCA-TS作用MCF-7细胞48 h后,细胞内线粒体膜电位、Ca 2+浓度和Cyt-C的表达水平均无显著变化;细胞内Caspase-8和Caspase-3的活性显著升高,而Caspase-12的活性均未见显著改变,Western blot检测细胞内Fas、FasL和FADD蛋白表达水平显著升高。综上说明OCA-TS诱导MCF-7细胞凋亡的作用可能是通过启动死亡受体途径而不是通过线粒体途径和内质网途径实现的。To investigate the molecular mechanism of apoptosis of human breast cancer MCF-7 cells induced by total saponins of Ornithogalum caudatum Ait(OCA-TS),MTT assay was used to detect the inhibitory effect of OCA-TS on the proliferation of MCF-7 cells and the changes of apoptosis morphology,membrane potential and apoptosis related protein content were observed.The results showed that OCA-TS could significantly inhibit the proliferation of human breast cancer MCF-7 cells(IC 50 was 93.17 g/mL),and the cells showed typical apoptotic morphology under electron microscopy.The results of flow cytometry showed that 48 hours after mca-7 cells were treated with different concentrations of OCA-TS,there was no significant change in mitochondrial membrane potential,Ca 2+concentration and Cyt-C expression level;the activities of Caspase-8 and Caspase-3 were significantly increased,but the activities of Caspase-12 were not significantly changed,and the expression levels of Fas,FasL and FADD protein were significantly increased by Western blot.In conclusion,the apoptosis of MCF-7 cells induced by OCA-TS may be realized by activating death receptor pathway rather than by mitochondrial pathway and endoplasmic reticulum pathway.

关 键 词：虎眼万年青 总皂苷 人乳腺癌MCF-7细胞 凋亡 抗肿瘤机制 

分 类 号：R285.5[医药卫生—中药学]