苹果炭疽病菌拮抗酵母菌的筛选与鉴定  

Screening and Identification of Antagonistic Yeast Against Colletotrichum gloeosporioides of Apple

作　　者：陈汝[1] 冉昆[1] 王传增[1] 薛晓敏[1] 王金政[1] CHEN Ru;RAN Kun;WANG Chuan-zeng;XUE Xiao-min;WANG Jin-zheng(Shandong Institute of Pomology,Tai’an 271000,China)

机构地区：[1]山东省果树研究所

出　　处：《河北农业科学》2019年第6期51-54,共4页Journal of Hebei Agricultural Sciences

基　　金：国家自然科学基金项目（31600021）;国家重点研发计划项目（2016YFD0201100,2017YFD0701400）;山东省重点研发计划项目（2017CXGC0210）;山东省农业科学院农业科技创新工程项目（CXGC2017D01）;东营市科技计划项目（2015GG0104）

摘　　要：为筛选出对苹果炭疽病菌具有高度抑制作用的拮抗菌,通过苹果炭疽病菌平板对峙试验,对从苹果果实表面分离到的19株酵母菌进行了筛选和鉴定。结果表明:酵母菌株YG3可明显抑制苹果炭疽病菌菌丝的生长,抑菌率为55.6%。通过核糖体26S D1/D2区核苷酸序列的比较,将酵母菌株YG3鉴定为Cryptococcus flavescens (GenBank NO.MF045447)。In order to screen antagonistic yeast with high inhibitory effect on Colletotrichum gloeosporioides of apple,19 yeast strains isolated from the surface of apple fruits were screened and identified by plate confrontation test. The results showed that the yeast strain YG3 could significantly inhibit the growth of apple anthracnose mycelia, and the inhibition rate was 55.6%. The yeast strain YG3 was identified as Cryptococcus flavescens(GenBank NO.MF045447) according to the nucleotide sequences of the ribosomal 26 S D1/D2 regions.

关 键 词：苹果炭疽病 拮抗酵母菌 筛选 鉴定 

分 类 号：Q939.95[生物学—微生物学]