运动疲劳对大鼠皮质M1区mGluR1和mGluR5蛋白表达的影响  

Effects of exercise fatigue on mGluR1 and mGluR5 expression in primary motor cortex of rats

作　　者：苏美华[1,2] 刘晓莉 乔德才[1] SU Mei⁃hua;LIU Xiaoli;QIAO Decai

机构地区：[1]北京师范大学体育与运动学院,北京100875 [2]闽南师范大学体育学院

出　　处：《中国康复医学杂志》2020年第1期5-9,共5页Chinese Journal of Rehabilitation Medicine

基　　金：福建省自然科学基金面上项目(2019J01754);福建省高校杰出青年人才计划项目(2005-B11504)

摘　　要：目的:疲劳是导致机体工作能力下降的重要原因,通过观察反复力竭运动大鼠皮质主运动区中mGluR1和mGluR5在运动前后的表达变化,探讨Ⅰ组代谢型谷氨酸受体介导运动疲劳产生的作用效应。方法:选用雄性Wistar大鼠32只,随机分为对照组(CG)、力竭即刻组(0EG)和运动后24h组(24EG),运动后48h组(48EG),每组8只,采用免疫组化染色和计算机图像分析系统检测并观察各组mGluR1和mGluR5表达的阳性细胞数和积分光密度(IOD)。结果:与对照组相比,力竭后即刻组及运动后24h组,大鼠皮质主运动区中mGluR1和mGluR5阳性细胞数量和IOD值均显著升高(P<0.05);运动后48h组mGluR1的蛋白表达较对照组仍有显著升高(P<0.05),而运动后48h组的mGluR5蛋白表达和对照组无显著差异(P>0.05)。结论:运动疲劳可导致大鼠中枢运动皮质代谢型谷氨酸受体的表达上调,不同亚型受体蛋白表达的时间效应不一样,提示mGluR1和mGluR5可能是中枢神经系统中介导运动疲劳产生的重要新型受体靶点。Objective: Fatigue is the crucial reason for the decline of working ability. By observing the expression changes of mGluR1 and mGluR5 in cortex M1 zone of rats pre and post exercise, the paper discussed the effects of mGluR1 and mGluR5 on the central nervous system induced by the exhaustive exercise.Method: Totally 32 male Wistar rats were randomly divided into four groups,CG, 0 EG, 24 EG and 48 EG, 8 rats for each. Meanwhile, immunohistochemistry(IMM) technique was used to investigate the expression of positive cell and integrated optical density(IOD) of mGluR1 and mGluR5 in different groups.Result: Compared with CG, the expression of positive cell and integrated optical density(IOD) of mGluR1 in0 EG and 24 EG both significantly increased(P<0.01). The expression of mGluR5 in 0 EG and 24 EG(P<0.01)also enhanced dramatically compared with CG. However, the expression of mGluR1 in the 48 EG was still higher than the CG(P<0.05), while the expression of mGluR5 in the 48 EG was not different from CG(P>0.05).Conclusion: Repeated exhaustive exercise could result in up-regulation the expression of mGluR1 and mGluR5 in cortex M1 zone of rats, which suggested that mGluR1 and mGluR5 with different time effects were another important receptors related with the production of exercise fatigue.

关 键 词：运动疲劳 大鼠 皮质 代谢型谷氨酸受体 M1区 

分 类 号：R493[医药卫生—康复医学]