Behavioral responses of potato tuber moth (Phthorimaea operculella) to tobacco plant volatiles  

作　　者：LI Xiang ZHANG Xiu-ge XIAO Chun GAO Yu-lin DONG Wen-xia 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,College of Plant Protection,Yunnan Agricultural University,Kunming 650201,P.R.China [2]College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,P.R.China [3]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第2期325-332,共8页农业科学学报（英文版）

基　　金：supported by the the National Natural Science Foundation of China (31560607);the National Key Research and Development Program of China (2018YFD0200802)

摘　　要：Potato tuber moth(PTM)Phthorimaea operculella,also known as tobacco splitworm,is an important pest of tobacco plants.The knowledge on the interaction of tobacco plant volatiles and PTM behavior is limited.To clarify the effect of tobacco plant volatiles on the orientation and oviposition behaviors of PTM and to identify potential compounds for PTM control,tobacco plant volatiles were collected by headspace collection method,and volatile compounds were identified by gas chromatography-mass spectrometry.Thirteen electrophysiological active compounds were screened by employing coupled gas chromatography-electroantennogram detection and from which nine available compounds were further verified by electroantennogram recording.cis-3-Hexen-1-ol showed significant attractant effect on the orientation behaviors of both male and female moths.Nonanal and decanal could only attract females,while octanal exhibited repellent effect on males.Oviposition selection experiment indicated that nonanal,decanal,decane and methyl hexadecanoate could stimulate the gravid females to lay more eggs,while octanal and 2,6-bis(1,1-dimethylenthyl)-4-methyl phenol inhibited their oviposition.Our study added more compounds to the list that could serve as potential PTM deterrents or attractants.Potato tuber moth(PTM) Phthorimaea operculella, also known as tobacco splitworm, is an important pest of tobacco plants. The knowledge on the interaction of tobacco plant volatiles and PTM behavior is limited. To clarify the effect of tobacco plant volatiles on the orientation and oviposition behaviors of PTM and to identify potential compounds for PTM control, tobacco plant volatiles were collected by headspace collection method, and volatile compounds were identified by gas chromatography-mass spectrometry. Thirteen electrophysiological active compounds were screened by employing coupled gas chromatography-electroantennogram detection and from which nine available compounds were further verified by electroantennogram recording. cis-3-Hexen-1-ol showed significant attractant effect on the orientation behaviors of both male and female moths. Nonanal and decanal could only attract females, while octanal exhibited repellent effect on males. Oviposition selection experiment indicated that nonanal, decanal, decane and methyl hexadecanoate could stimulate the gravid females to lay more eggs, while octanal and 2,6-bis(1,1-dimethylenthyl)-4-methyl phenol inhibited their oviposition. Our study added more compounds to the list that could serve as potential PTM deterrents or attractants.

关 键 词：Phthorimaea operculella tobacco volatiles electrophysiological response ORIENTATION OVIPOSITION 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]