Comparative transcriptome analysis of the effect of different heat shock periods on the unfertilized ovule in watermelon(Citrullus lanatus)  

作　　者：ZHU Ying-chun SUN De-xi DENG Yun AN Guo-lin LI Wei-hua SI Wen-jing LIU Jun-pu SUN Xiao-wu 

机构地区：[1]College of Horticulture,Hunan Agricultural University,Changsha 410128,P.R.China [2]Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第2期528-540,共13页农业科学学报（英文版）

基　　金：supported by the earmarked fund for China Agriculture Research System (CARS-25);the Fundamental Research Funds of the Chinese Academy of Agricultural Sciences (Y2018YJ15 and Y2019XK16-03);the Agricultural Science and Technology Innovation Program, Chinese Academy of Agricultural Sciences (CAAS-ASTIP2018-ZFRI);the National Key R&D Program of China (2018YFD0201310)

摘　　要：In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding.However,because of low repeatability,embryoid induction rate and quality,the molecular mechanisms remain poorly understood.Heat shock treatment can promote the transformation of the gametophytic pathway into the sporophyte pathway,which induces the occurrence of haploid.In this study,unfertilized ovaries were heat shocked for 0 h(A0)before flowering and for 0 h(A1),4 h(A3),8 h(A5),12 h(A7),and 24 h(A8),respectively,at 37℃at the first day of the flowering stage.The ovule enlargement rate was increased from 0%at 25℃to 96.8%at 37℃(24 h treatment).Thus,we aimed to investigate the gene expression patterns in unfertilized ovules of watermelon after different periods of heat shock by using RNA-Seq technology.The results showed that compared with A3,A5,A7,and A8,the biosynthesis of amino acid,glycine,serine and threonine metabolic pathways in A1 has changed significantly.This indicated that heat shock treatment affected the synthesis and transformation of amino acids during ovule expansion.The transcriptome data suggested gene expressions of ovule growth were significantly changed by heat-specific influences.The results provide new information on the complex relationship between in vitro gynogenesis and temperature.This provides a basis for further study of the mechanism of heat shock affecting the expansion of watermelon ovule.In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding. However, because of low repeatability, embryoid induction rate and quality, the molecular mechanisms remain poorly understood. Heat shock treatment can promote the transformation of the gametophytic pathway into the sporophyte pathway, which induces the occurrence of haploid. In this study, unfertilized ovaries were heat shocked for 0 h(A0) before flowering and for 0 h(A1), 4 h(A3), 8 h(A5), 12 h(A7), and 24 h(A8), respectively, at 37°C at the first day of the flowering stage. The ovule enlargement rate was increased from 0% at 25°C to 96.8% at 37°C(24 h treatment). Thus, we aimed to investigate the gene expression patterns in unfertilized ovules of watermelon after different periods of heat shock by using RNA-Seq technology. The results showed that compared with A3, A5, A7, and A8, the biosynthesis of amino acid, glycine, serine and threonine metabolic pathways in A1 has changed significantly. This indicated that heat shock treatment affected the synthesis and transformation of amino acids during ovule expansion. The transcriptome data suggested gene expressions of ovule growth were significantly changed by heat-specific influences. The results provide new information on the complex relationship between in vitro gynogenesis and temperature. This provides a basis for further study of the mechanism of heat shock affecting the expansion of watermelon ovule.

关 键 词：WATERMELON heat shock unfertilized ovule ovule enlargement transcription factors 

分 类 号：S651[农业科学—果树学]