Putrescine, spermidine, and spermine play distinct roles in rice salt tolerance  

作　　者：Md Azizul ISlam PANG Jin-huan MENG Fan-wei LI Ya-wen XU Ning YANG Chao LIU Jun 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Sciences(CAS),Beijing 100101,P.R.China [2]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,P.R.China [3]Department of Biotechnology and Genetic Engineering,Faculty of Applied Science and Technology,Islamic University,Kushtia 7003,Bangladesh

出　　处：《Journal of Integrative Agriculture》2020年第3期643-655,共13页农业科学学报（英文版）

基　　金：supported by the National Key R&D Program(2017YFD0200900);the Natural Science Foundation of China(31570252 and 31601629);the Chinese Academy of Sciences(CAS)and the World Academy of Sciences(TWAS)Presidents Fellowship for supporting Md Azizul Islam as a doctoral student(2015CTF068)

摘　　要：Polyamines(PAs) play diverse roles in plant growth and development,as well as responses to environmental stimuli.In this study,the effects of PAs on rice salt tolerance were investigated.Salt stress resulted in the alteration of endogenous PAs levels in rice roots and leaves,where spermidine(Spd) and spermine(Spm) contents were increased,and putrescine(Put) content was decreased.RT-qPCR analysis revealed that PAs biosynthesis-related genes ADC1,ODC,and Arginase were significantly downregulated by salt treatment;however,SAMDC transcription was significantly upregulated.Exogenous Spm enhanced rice salt tolerance remarkably;however,exogenous Put and Spd undermined rice salt tolerance.Transgenic rice plants overexpressing SAMDC display a higher ratio of Spm/(Put+Spd) and enhanced salt tolerance.Salt stress also increased polyamine oxidase activities in rice,resulting in elevated reactive oxygen species(ROS) production.Our findings revealed that accumulation of Put and Spd substantially reduced salt tolerance in rice,likely by facilitating ROS production;whereas,conversion of Put and Spd to Spm contributes to rice salt tolerance.Polyamines(PAs) play diverse roles in plant growth and development, as well as responses to environmental stimuli. In this study, the effects of PAs on rice salt tolerance were investigated. Salt stress resulted in the alteration of endogenous PAs levels in rice roots and leaves, where spermidine(Spd) and spermine(Spm) contents were increased, and putrescine(Put) content was decreased. RT-qPCR analysis revealed that PAs biosynthesis-related genes ADC1, ODC, and Arginase were significantly downregulated by salt treatment; however, SAMDC transcription was significantly upregulated. Exogenous Spm enhanced rice salt tolerance remarkably; however, exogenous Put and Spd undermined rice salt tolerance. Transgenic rice plants overexpressing SAMDC display a higher ratio of Spm/(Put+Spd) and enhanced salt tolerance. Salt stress also increased polyamine oxidase activities in rice, resulting in elevated reactive oxygen species(ROS) production. Our findings revealed that accumulation of Put and Spd substantially reduced salt tolerance in rice, likely by facilitating ROS production; whereas, conversion of Put and Spd to Spm contributes to rice salt tolerance.

关 键 词：RICE polyamines NaCl polyamine oxidase guazatine 

分 类 号：S511[农业科学—作物学]