The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage  

作　　者：GUO Gang SHEN Chen LIU Qiang ZHANG Shuan-lin SHAO Tao WANG Cong WANG Yongxin XU Qing-fang HUO Wen-jie 

机构地区：[1]College of Animal Sciences and Veterinary Medicines,Shanxi Agricultural University,Taigu 030801,P.R.China [2]Institute of Ensiling and Processing of Grass,Nanjing Agricultural University,Nanjing 210095,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第3期838-847,共10页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31502015,31672488);the Natural Science Foundation of Shanxi Province of China(2015021162)

摘　　要：The objective of this study was to investigate the effect of lactic acid bacteria(LAB) inoculums on fermentation quality and in vitro digestibility of corn stover silage.Corn stover was ensiled without(control) or with Lactobacillus plantarum(LP),Enterococcus faecalis(EF),and Enterococcus mundtii(EM) for 45 days.The fermentation characteristics were assessed,and subsequent in vitro dry matter digestibility(DM-D),neutral detergent fiber digestibility(NDF-D),volatile fatty acids(VFA),methane(CH4) production,cellulolytic bacteria proportions and their activities per corn stover silage were also determined.There was no significant difference(P>0.05) among the silage pH,lactic acid,crude protein(CP),water soluble carbohydrates(WSC) and lignocelluloses contents of different treatments.The relative proportions of Ruminococcus flavefaciens and Fibrobacter succinogenes,carboxymethyl-ocellulose and β-glycosidase activities,DM-D,NDF-D,and VFA production of in vitro incubation was higher(P<0.05) for silages inoculated with LP and EF than those of the control silage.Silage inoculated with LP showed the lowest(P<0.05) CH4 production per unit yield of VFA,which was positively corresponded to the lowest(P<0.05) ratio of acetate to propionate.In summary,the ensiling fermentation quality and subsequent utilization of corn stover silage were efficiently improved by inoculated with L.plantarum.The objective of this study was to investigate the effect of lactic acid bacteria（LAB） inoculums on fermentation quality and in vitro digestibility of corn stover silage. Corn stover was ensiled without（control） or with Lactobacillus plantarum（LP）, Enterococcus faecalis（EF）, and Enterococcus mundtii（EM） for 45 days. The fermentation characteristics were assessed, and subsequent in vitro dry matter digestibility（DM-D）, neutral detergent fiber digestibility（NDF-D）, volatile fatty acids（VFA）, methane（CH4） production, cellulolytic bacteria proportions and their activities per corn stover silage were also determined. There was no significant difference（P>0.05） among the silage pH, lactic acid, crude protein（CP）, water soluble carbohydrates（WSC） and lignocelluloses contents of different treatments. The relative proportions of Ruminococcus flavefaciens and Fibrobacter succinogenes, carboxymethyl-ocellulose and β-glycosidase activities, DM-D, NDF-D, and VFA production of in vitro incubation was higher（P<0.05） for silages inoculated with LP and EF than those of the control silage. Silage inoculated with LP showed the lowest（P<0.05） CH4 production per unit yield of VFA, which was positively corresponded to the lowest（P<0.05） ratio of acetate to propionate. In summary, the ensiling fermentation quality and subsequent utilization of corn stover silage were efficiently improved by inoculated with L. plantarum.

关 键 词：corn stover in vitro digestibility lactic acid bacteria SILAGE 

分 类 号：S816.53[农业科学—饲料科学]