Immobilization of functional nano-objects in living engineered bacterial biofilms for catalytic applications  

作　　者：Xinyu Wang Jiahua Pu Yi Liu Fang Ba Mengkui Cui Ke Li Yu Xie Yan Nie Qixi Mi Tao Li Lingli Liu Manzhou Zhu Chao Zhong 

机构地区：[1]Division of Materials and Physical Biology,School of Physical Science and Technology,ShanghaiTech University,Shanghai 201210,China [2]Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200050,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China [5]Shanghai Institute for Advanced Immunochemical Studies(SIAIS),ShanghaiTech University,Shanghai 201210,China [6]College of Chemistry&Chemical Engineering,Anhui University,Hefei 230039,China

出　　处：《National Science Review》2019年第5期929-943,共15页国家科学评论（英文版）

基　　金：supported by the Science and Technology Commission of Shanghai Municipality(17JC1403900);the National Natural Science Foundation of China(31570972);the 2016 Open Financial Fund of Qingdao National Laboratory for Marine Science and Technology(QNLM2016ORP0403)for C.Z.;funding support from Shanghai Tech University;the 1000 Youth Talents Program,granted by the Chinese Central Government

摘　　要：Nanoscale objects feature very large surface-area-to-volume ratios and are now understood as powerful tools for catalysis,but their nature as nanomaterials brings challenges including toxicity and nanomaterial pollution.Immobilization is considered a feasible strategy for addressing these limitations.Here,as a proof-of-concept for the immobilization of nanoscale catalysts in the extracellular matrix of bacterial biofilms,we genetically engineered amyloid monomers of the Escherichia coli curli nanofiber system that are secreted and can self-assemble and anchor nano-objects in a spatially precise manner.We demonstrated three scalable,tunable and reusable catalysis systems:biofilm-anchored gold nanoparticles to reduce nitro aromatic compounds such as the pollutant p-nitrophenol,biofilm-anchored hybrid Cd0.9Zn0.1S quantum dots and gold nanoparticles to degrade organic dyes and biofilm-anchored CdSeS@ZnS quantum dots in a semi-artificial photosynthesis system for hydrogen production.Our work demonstrates how the ability of biofilms to grow in scalable and complex spatial arrangements can be exploited for catalytic applications and clearly illustrates the design utility of segregating high-energy nano-objects from injury-prone cellular components by engineering anchoring points in an extracellular matrix.Nanoscale objects feature very large surface-area-to-volume ratios and are now understood as powerful tools for catalysis, but their nature as nanomaterials brings challenges including toxicity and nanomaterial pollution.Immobilization is considered a feasible strategy for addressing these limitations.Here, as a proof-of-concept for the immobilization of nanoscale catalysts in the extracellular matrix of bacterial biofilms, we genetically engineered amyloid monomers of the Escherichia coli curli nanofiber system that are secreted and can self-assemble and anchor nano-objects in a spatially precise manner.We demonstrated three scalable, tunable and reusable catalysis systems: biofilm-anchored gold nanoparticles to reduce nitro aromatic compounds such as the pollutant p-nitrophenol, biofilm-anchored hybrid Cd0.9Zn0.1S quantum dots and gold nanoparticles to degrade organic dyes and biofilm-anchored CdSeS@ZnS quantum dots in a semi-artificial photosynthesis system for hydrogen production.Our work demonstrates how the ability of biofilms to grow in scalable and complex spatial arrangements can be exploited for catalytic applications and clearly illustrates the design utility of segregating high-energy nano-objects from injury-prone cellular components by engineering anchoring points in an extracellular matrix.

关 键 词：nanoscale catalyst IMMOBILIZATION semi-artificial PHOTOSYNTHESIS LIVING catalysis bio-inorganic hybrid system hydrogen production 

分 类 号：O643.36[理学—物理化学] TB383.1[理学—化学]