沙棘3-磷酸甘油脱氢酶基因生物信息学及表达分析  

Gene Expression and Bioinformatics Analysis of HrGPD1 in Sea Buchthorn(Hippophae L.)

作　　者：刘景 丁健 阮成江 杜维 张莞晨 韩平 Liu Jing;Ding Jian;Ruan Chengjiang;Du Wei;Zhang Wanchen;Han Ping(Key Laboratory of Biotechnology and Bioresources Utilization,Ministry of Education,Institute of Plant Resources,Dalian Minzu University,Dalian,116600)

机构地区：[1]大连民族大学资源植物研究所生物技术与资源利用教育部重点实验室

出　　处：《分子植物育种》2020年第2期409-415,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31800574; 31570681);大连市科协高校创新助力联合体项目(大科协发[2018]65号);大连民族大学自主科研基金学科团队项目(0919110127)和大连民族大学人才引进科研项目(0701110152)共同资助

摘　　要：沙棘果肉和种子组织中均富含生物活性油。3-磷酸甘油脱氢酶(GPD1)是促进油脂合成前体甘油-3-磷酸(G3P)合成的限速酶。本研究对沙棘HrGPD1基因进行生物信息学分析(氨基酸序列,保守域,亚细胞定位等),及其在沙棘不同组织(果肉和种子)中的表达情况。研究发现:HrGPD1开放阅读框为1 143 bp,编码380个氨基酸,无信号肽和跨膜结构域,存在多个磷酸化位点,定位于质体中,蛋白高级结构以α-螺旋为主,该蛋白序列与桑、梅、烟草和拟南芥的GPD表现出较高的序列相似性。qRT-PCR分析发现,HrGPD1基因在沙棘品系‘新俄3号’成熟种子和果肉中的相对表达量分别为0.52和1.35,与二者的含油率高低规律相一致。这为进一步研究HrGPD1基因在沙棘生物活性油合成过程中的作用机理提供科学依据。Sea buckthorn(Hippophae L.), is a species of oil-producing shrubs plant in the family Elaeagnaceae,also known as high functional oil store in seed and berry tissues. Glycerol-3-phosphate(G3 P) is a primary substrate for triacylglycerols(TAG) synthesis, and G3 P can be catalysed by glycerol-3-phosphate dehydrogenase(GPD1)from glycerol. In the present study, we obtained a HrGPD1 gene firstly base on the transcriptome sequencing data from H. rhamnoides. Bioinformatic analysis(amino acid sequence, conserved domain, subcellular localization,phylogenetic tree, etc) of HrGPD1 and its gene expression analysis between sea buckthorn tissues(berry pulp and seed) by q RT-PCR indicated that open reading frame of HrGPD1 was 1 143 bp, encoding 380 amino acid residues,no transmembrane regions and signal peptide, contained multiple phosphorylation sites, and located to the chloroplast. In addition, the secondary structure was mainly based on alpha helix. The amino acid sequence of Hr GPD1 shared high homology with GPD from Morus notabilis, Prunus mume, Nicotiana tabacum and Arabidopis thaliana. qRT-PCR results revealed that HrGPD1 gene was significantly more highly expressed in pulp(1.35) than in seed(0.52) of sea buckthorn line ’Xin’e 3’, which is consistent with the data from the oil contents of pulp and seed. These results provided scientific basis for understanding the molecular regulatory mechanism of HrGPD1 gene in sea buckthorn oil synthesis pathway.

关 键 词：沙棘(Hippophae L.) HrGPD1基因 生物信息学分析 基因表达分析 

分 类 号：S793.6[农业科学—林木遗传育种]