2种甜玉米自交系幼胚再生体系的建立  

Establishment of Sweet Maize Immature Embryo Regeneration System for Two Inbred Lines

作　　者：高玉尧[1,3] 许文天 胡小文[1,3] 徐磊[1,3] 刘洋 Gao Yuyao;Xu Wentian;Hu Xiaowen;Xu Lei;Liu Yang(Zhanjiang Experiment Station,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,524013;South Subtropical Crop Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,524091;Guangdong Engineering Technology Research Center for Dryland Water-saving Agriculture,Zhanjiang,524013)

机构地区：[1]中国热带农业科学院湛江实验站,湛江524013 [2]中国热带农业科学院南亚热带作物研究所,湛江524091 [3]广东省旱作节水农业工程技术研发中心,湛江524013

出　　处：《分子植物育种》2020年第2期501-506,共6页Molecular Plant Breeding

基　　金：广东省自然科学基金项目(2017A030307032; 2016A030307007)资助

摘　　要：本试验研究不同基因型、幼胚大小、培养基类型及培养基添加物对2种甜玉米(2-105和2-118)自交系幼胚愈伤及胚性愈伤组织诱导率的影响。结果表明,基因型、培养基类型、幼胚大小及不同浓度脯氨酸和2,4-D处理对愈伤及胚性愈伤诱导率影响差异均显著。2种基因型最适宜的基本培养基为N6培养基,且2-118的愈伤及胚性愈伤诱导率显著高于2-105。幼胚在1.5~2.0 mm时,2-105和2-118的愈伤及胚性愈伤诱导率均最高,分别在77%和51%以上。2-105和2-118在脯氨酸浓度分别为650 mg/L、750 mg/L时幼胚的愈伤及胚性愈伤诱导率最高,且显著高于其它2个水平。2种基因型在2.5 mg/L的2,4-D诱导下愈伤及胚性愈伤诱导率均最高,且均显著高于其它2个水平。本研究获得了2种甜玉米自交系的再生体系,为甜玉米遗传转化和分子育种提供基础。In this study, the effects of different genotypes, immature embryos size, medium types, medium additions on induction rate of callus and embryogenic callus in sweet maize for 2 inbred lines were investigated.The results showed that the genotypes, medium types, immature embryos size and different treatments of proline and 2,4-D had significant effects on the induction rate of callus and embryogenic callus(p<0.05). The induction rates of callus and embryogenic callus from 2-118 were significantly higher than that from 2-105, and the best basal medium for the 2 genotypes was N6 medium. Moreover, the induction rates of callus and embryogenic callus both from the 2-105 and 2-118 were highest when the immature embryos size were 1.5~2.0 mm, more than 77%and 85%, respectively. Besides, the induction rates of callus and embryogenic callus both from the 2-105 and2-118 were highest when the concentrations of the proline were 650 mg/L and 750 mg/L separately and the concentrations of the 2,4-D were 2.5 mg/L. Regeneration system of 2 inbred lines were obtained in this study which would provide important foundation for genetic transformation and molecular breeding of sweet maize.

关 键 词：甜玉米(Zea mays L.saccharata Sturt.) 幼胚 愈伤组织 再生体系 

分 类 号：S513[农业科学—作物学]