正交法优化超临界CO2流体萃取糖茶藨籽油工艺的探究  

Study on Optimization of Supercritical CO2 Fluid Extraction of Ribes himalense Seed Oil by Orthogonal Method

作　　者：孙晴 徐文华[1] 杨海文 薛长福 张世玺 韩才邦 Sun Qing;Xu Wenhua;Yang Haiwen;Xue Changfu;Zhang Shixi;Han Caibang(Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources,Northwest Institute of Plateau Biology Chinese Academy of Science,Xining,810008;Marke River Forestry Bureau,Qinghai Province,Guoluo,814399;University of Chinese Academy of Sciences,Beijing,100049)

机构地区：[1]中国科学院西北高原生物研究所,青海省青藏高原特色生物资源研究重点实验室,西宁810008 [2]青海省玛可河林业局,果洛814399 [3]中国科学院大学,北京100049

出　　处：《分子植物育种》2020年第2期602-607,共6页Molecular Plant Breeding

基　　金：青海省应用基础研究项目(2017-ZJ-749);青海省重点研发与转化计划项目(2019-NK-C04)共同资助

摘　　要：采用超临界CO2萃取技术,经单因素试验研究投料量、粉碎度、萃取压力、萃取温度和萃取时间对糖茶藨籽油萃取率的影响;以糖茶藨籽油萃取率为指标,经正交试验选出最佳萃取工艺条件;通过GC-MS对提取的糖茶藨籽油进行组分分析。结果表明,最佳糖茶藨籽油的萃取条件为投料量400 g、粉碎度35目、压力35 MPa、温度45℃、时间50 min,在此条件下糖茶藨籽提油率为23.23%;超临界CO2流体萃取的糖茶藨籽油是澄明的黄色油状液体,用GC-MS检测不饱和脂肪酸含量达85%以上,其中亚油酸含量为29.57%,糖茶藨籽油酸价是1.10 mg/g,过氧化值是2.70 mmol/kg。本研究得出该工艺条件可为糖茶藨籽油的提取工艺提供了实验支持和理论依据。The effects of inventory rating, pulverization degree, extraction pressure, extraction temperature and extraction time on the extraction rate of Ribes himalense seed oil were studied by single factor experiment using supercritical CO2 fluid extraction technique. The extraction rate of Ribes himalense seed oil was the index. The optimal process conditions were screened by orthog orthogonal test;the components of the extracted Ribes himalense seed oil were analyzed by GC-MS. The results showed that the optimum conditions were as follows:feeding amount 400 g, pulverization degree 35 mesh, extraction pressure 35 MPa, extraction temperature 45℃,extraction time 50 min. Under this condition, the extraction rate of Ribes himalense was 23.23%;supercritical CO2 fluid extracted Ribes himalense seed oil is a clear yellow oily liquid. The content of unsaturated fatty acid is more than 85% by GC-MS, wherein the linoleic acid content is 29.57%, and the acid value of the Ribes himalense seed oil is 1.10 mg/g. The peroxide value was 2.70 mmol/kg. This study concluded that the process conditions are feasible and provided experimental support and theoretical basis for the extraction process of Ribes himalense seed oil.

关 键 词：超临界CO2萃取  糖茶藨籽油  工艺优化  GC-MS 

分 类 号：TQ028.32[化学工程] TQ460.1