电针“夹脊”穴对坐骨神经慢性损伤大鼠镇痛后效应的影响  

Electroacupuncture at “Jiaji” (EX-B2) induced down-regulation of OX-42 and P2X4 in lumbar spinal cord contributes to its analgesic after-effect in rats with chronic constriction injury

作　　者：黄华 陈慧娥 余文英 黄明愉 吴倩雯 林丽莉[1] HUANG Hua;CHEN Hui-e;YU Wen-ying;HUANG Ming-yu;WU Qian-wen;LIN Li-li(College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学针灸学院

出　　处：《针刺研究》2020年第1期40-45,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81704194);福建省教育厅产学研项目(No.JK2016018、JAT160221)

摘　　要：目的:观察不同时间点电针"夹脊"穴对坐骨神经慢性压迫性损伤(CCI)大鼠脊髓小胶质细胞特异表达的补体受体-3的单克隆抗体(OX-42)及嘌呤受体P2X4(P2X4)蛋白表达的影响,探讨电针干预神经病理性疼痛中可能的后效应机制。方法:SD大鼠随机分为空白组、模型组、电针后即刻组、电针后0.5h组、电针后1h组、电针后2h组、电针后4h组、电针后12h组、电针后24h组,每组各6只。模型组与各电针组采用结扎坐骨神经法建立大鼠CCI模型。各电针组于造模7d后电针双侧L3、L5夹脊穴20min,分别于电针后即刻及电针后0.5、1、2、4、12、24h取材,空白组与模型组仅固定20min后取材。观察各组大鼠干预前后热痛阈值的变化并用免疫组织化学法检测各组大鼠脊髓腰膨大OX-42、P2X4蛋白的表达情况。结果:造模7d后(干预前),与空白组比较,模型组和各电针组的热痛阈值明显下降(P<0.01);干预后与模型组比较,电针后即刻组、电针后0.5h组、电针后1h组及电针后2h组大鼠的热痛阈值明显升高(P<0.05)。与空白组比较,模型组大鼠脊髓腰膨大OX-42、P2X4蛋白表达明显升高(P<0.01);与模型组比较,电针后即刻组、电针后0.5h组、电针后1h组及电针后2h组大鼠的脊髓腰膨大OX-42蛋白表达明显下降(P<0.01),电针后即刻组、电针后0.5h组及电针后1h组大鼠脊髓腰膨大的P2X4蛋白表达明显下降(P<0.01)。结论:电针"夹脊"穴可明显下调CCI大鼠脊髓OX-42、P2X4蛋白的表达,提高大鼠热痛阈,在电针后0.5h下调最明显,其镇痛后效应可延续2h。Objective To investigate the effect of electroacupuncture(EA)at"Jiaji"(EX-B2)at different time points on the expression of OX-42(a monoclonal antibody with specific expression of complement receptor-3 in spinal microglial cells)and purinergic receptor P2X4(P2X4)in rats with chronic constriction injury(CCI)of the sciatic nerve,as well as the possible aftereffect mechanism of EA analgesia in neuropathic pain.Methods Sprague-Dawley rats were randomly divided into blank group,model group,immediately after EA group,0.5-hour after EA group,1-hour after EA group,2-hour after EA group,4-hour after EA group,12-hour after EA group,and 24-hour after EA group,with 6 rats in each group.The rats in the model group and the EA groups were used to establish a model of CCI-induced neuropathic pain,and those in the immediately after EA group and the 0.5,1,2,4,12 and 24 hours after EA groups were treated with EA at bilateral L3 and L5"Jiaji"points for 20 min after 7 dof modeling.Samples were collected immediately and at 0.5,1,2,4,12,and 24 hours after EA,and for the rats in the blank group and the model group,samples were collected after fixation the rats for 20 min.Heat pain threshold was observed before and after intervention,and immunohistochemistry was used to measure the protein expression of OX-42 and P2X4 in the spinal cord lumbar enlargement.Results After 7 days of modeling(before intervention),compared with the blank group,the heat pain threshold had a significant reduction in the model group and the EA groups(P<0.01).Compared with the model group after intervention,the immediately after EA group and the 0.5,1 and 2 hours after EA groups had a significant increase in heat pain threshold(P<0.05).Compared with the model group,immediately after EA,the 0.5,1 and 2 hours after EA groups had a significant reduction in the protein expression of OX-42(P<0.01),and immediately after EA,the 0.5 and 1 hour after EA groups had a significant reduction in the protein expression of P2X4(P<0.01).Conclusion EA at"Jiaji"points can significant

关 键 词：慢性压迫性损伤 电针 小胶质细胞特异表达的补体受体-3的单克隆抗体 嘌呤受体P2X4 后效应 

分 类 号：R245.97[医药卫生—针灸推拿学]