KRTCAP3基因多态性与细毛羊产毛及生长性状的相关性分析  

Association Analysis of Polymorphism of KRTCAP3 Gene with Wool Production and Growth Performance in Fine Wool Sheep(Ovis aries)

作　　者：王丽[1] 胡瑞雪 李发弟[1] 乐祥鹏[1] WANG Li;HU Rui-Xue;LI Fa-Di;YUE Xiang-Peng(State Key Laboratory of Grassland Agro-ecosystems/Key Laboratory of Grassland Livestock Industry Innovation/Ministry of Agriculture and Rural Affairs/Engineering Research Center of Grassland Industry,Ministry of Education/College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]兰州大学草地农业生态系统国家重点实验室/农业农村部草牧业创新重点实验室/草地农业教育部工程研究中心/草地农业科技学院,兰州730020 [2]华中农业大学动物科技学院,武汉430070

出　　处：《农业生物技术学报》2020年第1期84-91,共8页Journal of Agricultural Biotechnology

基　　金：甘肃省重点研发计划(17YF1NA066);长江学者和创新团队发展计划(IRT_17R50);国家肉羊产业技术体系(CARS-38)

摘　　要：角化细胞相关蛋白3基因(keratinocyte-associated protein 3,KRTCAP3)主要调节角蛋白表达进而影响机体皮肤、指甲和毛发的生长发育。为了解KRTCAP3基因在‘南甘’杂交绵羊(Ovis aries)群体中的遗传变异及其与产毛和生长性状之间的关系,本研究以南非肉用美利奴(Ovis aries)(♂)×甘肃高山细毛羊(♀)(‘南甘’)及南非肉用美利奴(♂)ב南甘’(♀)(‘南南甘’)杂种羊为研究对象,采用DNA混合池测序结合SNPscanTM高通量SNP分型技术对KRTCAP3的基因全长进行SNP扫描,同时分析SNP位点与杂种羊产毛和生长性状的相关性。结果表明,在KRTCAP3基因第2内含子上发现1个g.34287851C>G的SNP位点,鉴定到CC、CG和GG 3种基因型,其基因型频率分别为0.35、0.52和0.13,C和G等位基因频率分别为0.61和0.39。遗传参数分析发现,杂合度(heterozygosity,He)、纯合度(homozygosity,Ho)、有效等位基因数(effective number of alleles,Ne)和多态信息含量(polymorphic information content,PIC)分别为0.47、0.53、1.90和0.36,属于中度多态(0.25G位点可作为分子标记辅助选育的候选分子标记,在细毛羊品种选育和培育肉毛兼用新品种中利用,加快育种进程。The keratinocyte-associated protein 3 gene(KRTCAP3)affects the growth of skin,nails and hair by regulating keratin.In order to uncover the genetic variation in the KRTCAP3 gene and its genetic roles in wool production and growth performance in nangan(South African Mutton Merino(♂)×Gansu alpinefinewool(♀))and nannangan(South African Mutton Merino(♂)×nangan(♀))crossbred sheep breed.DNA pool sequencing was used to scan the potential SNPs within the full-length DNA of KRTCAP3 gene in AF×MF population.The correlation between SNP locus and hair growth and growth traits of hybrid sheep was also analyzed.Six hundred crossbred sheep were individually genotyped by SNPscanTMmethod.Then,the association analysis between SNP locus and wool production and growth performance was implemented.The results showed that one SNP(g.34287851 C>G)was identified in intron2.Three genotypes,CC,CG and GG,in this locus were found in AF×MF crossbreed sheep population.The genotypic frequencies of CC,CG and GG were 0.35,0.52 and 0.13,respectively.The allelic frequencies of C and G were 0.61 and 0.39,respectively.The population genetic parameters analyses showed that the homozygosity(Ho),heterozygosity(He)and effective allele number(Ne)were 0.53,0.47 and 1.90,respectively.The polymorphism information contents(PIC)was 0.36 and suggested that this SNP was moderate diversity(0.25G was significantly associated with the wool length of shoulder and side,wool length of thigh and notum,crimpness and crimp recovery(P<0.05 or P<0.01).The association analysis of polymorphisms in KRTCAP3 gene with growth performance revealed that g.34287851 C>G was significantly associated with tube circumference and chest girth(P<0.05 or P<0.01).In summary,the g.34287851 C>G site in KRTCAP3 gene could be used as a useful molecular marker for selection wool traits and growth performance.This study provides a molecular basis for utilization of fine wool resources and accelerates the breeding progress.

关 键 词：角化细胞相关蛋白3基因(KRTCAP3) 遗传多态性 产毛性状 

分 类 号：S826[农业科学—畜牧学]