迁徙候鸟产气荚膜梭菌的分离鉴定与特性分析  

Isolation and specificity analysis of Clostridium perfringens from migratory birds

作　　者：司南 祝令伟[2] 景洁 梁冰[2] 王莹 纪雪[2] 郑林 陈萍[1] 郭学军[2] SI Nan;ZHU Ling-wei;JING Jie;LIANG Bing;WANG Ying;JI Xue;ZHENG Lin;CHEN Ping;GUO Xue-jun(Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China;Key Laboratory of Jilin Province for Zoonosis Prevention and Control,Institute of Military Veterinary Medicine,the Academy of Military Medical Sciences,Changchun 130122,China)

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]军事医学科学院军事兽医研究所/吉林省人兽共患病预防与控制重点实验室,吉林长春130122

出　　处：《中国兽医学报》2020年第1期129-134,共6页Chinese Journal of Veterinary Science

基　　金：国家重点研发计划资助项目(2016YFD0501305,2016YFC1201602)

摘　　要：产气荚膜梭菌是一种人兽共患病原菌,可通过多种途径感染人类。本研究从迁徙候鸟样品中对产气荚膜梭菌进行分离鉴定,并进行毒力基因检测、分型、生物被膜形成能力鉴定及耐药性测定。结果从549份健康候鸟粪便样品中分离细菌42株,35只死亡候鸟病料样品中分离细菌11株,通过PCR方法共鉴定A型菌48株,E型菌5株,所有菌株均未检出β2毒素、肠毒素和NetB毒素基因,30株经测定能够形成生物膜;分离菌株对黏菌素、杆菌肽、克林霉素和红霉素的耐药率在40%以上,所有菌株均对复方新诺明敏感。结论得出迁徙候鸟携带产气荚膜梭菌的比例较低,但是对部分抗生素的高耐药性值得关注。Clostridium perfringens is a zoonotic pathogen,which can infect people by many ways.In this study,forty-two strains from 549 migratory birds fecal and 11 from 35 dead migratory bird samples were recovered,respectively.Their virulence genes were detected,biofilm formation and drug resistance of these strains were evaluated.Forty-eight toxin A strains and 5 toxin E strains were confirmed by PCR,however,no β2 toxinen gene,enterotoxin gene and netB toxin genes were detected in all strains;Thirty strains could form biofilms.The percentage of strains reducing sensitivity to myxin,bacitracin,clindamycin and erythromycin were more than 40%.All strains were sensitive to compound sulfamethoxine.In conclusion,migratory birds carrying C.perfringens were not popular,but strains with high rate of resistance to some antibiotics need to be paid more attention.

关 键 词：迁徙候鸟 产气荚膜梭菌 毒素分型 耐药性 

分 类 号：S182[农业科学—农业基础科学]