精准高效外源DNA整合技术研究进展  

Advances on Efficient and Precise Targeted Foreign DNA Integration

作　　者：吴志胜 傅高惠 罗文骏 刘俊杰 陈慧芳 白银山 WU Zhi-sheng;FU Gao-hui;LUO Wen-jun;LIU Jun-jie;CHEN Hui-fang;BAI Yin-shan(School of Life Science and Engineering,Foshan University,Foshan 528231)

机构地区：[1]佛山科学技术学院生命科学与工程学院

出　　处：《生物技术通报》2020年第3期29-37,共9页Biotechnology Bulletin

基　　金：广东省畜禽疫病防治研究重点实验室基金项目(YDWS1902);佛山科学技术学院高层次人才启动项目(gg040969)

摘　　要：精准高效整合技术是将外源DNA片段插入到目的细胞基因组特定位置的一项转基因技术。早期通过细胞基因组与外源DNA同源序列发生重组来完成靶向整合的目的。伴随基因编辑技术发展,特别是CRISPR/Cas9技术的出现,精准高效的外源DNA整合技术日益成熟,广泛应用到功能基因组学、转基因动物及遗传疾病治疗的研究中。围绕基因编辑研究进展、引导RNA修饰技术、单碱基整合技术、转座子技术和外源DNA整合效率等方面对精准靶向和高效整合技术进行综述。Precise and efficient target gene integration is a transgenic technology that inserts foreign DNA into the aimed position of cell genome.Integration of the target gene is achieved by the recombination of the homologous sequences of both cell genomic DNA and foreign DNA in the early stage.With the development of gene editing technology,especially the emergence of CRISPR/Cas9,the efficient and precise technology of foreign DNA integration becomes more and more mature and could be widely used in the researches on functional genomics,transgenic animals and genetic disease treatment.Here,we reviewed the research advances on precise target and efficient integration technologies such as gene editing,guided RNA modification technology,single base integration technology,transposon technology,and foreign DNA typing efficiency.

关 键 词：外源DNA整合 精准高效 CRISPR/Cas9 单碱基整合 转座子技术 

分 类 号：Q523[生物学—生物化学] Q78