动物转基因高效表达策略研究进展  

Strategies for Efficient Exogenous Gene Expression in Transgenic Animals

作　　者：赵旭东 黄永志 毕延震[2] 董发明[1] ZHAO Xu-dong;HUANG Yong-zhi;BI Yan-zhen;DONG Fa-ming(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023;Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064)

机构地区：[1]河南科技大学动物科技学院,洛阳471023 [2]动物胚胎工程及分子育种湖北省重点实验室湖北省农业科学院畜牧兽医研究所,武汉430064

出　　处：《生物技术通报》2020年第3期45-53,共9页Biotechnology Bulletin

基　　金：转基因生物新品种培育重大专项(2016ZX08010003-006);动物胚胎工程及分子育种湖北省重点实验室开放课题(KLAEMB-2018-03);湖北省自然科学基金创新群体(2018CFA014)

摘　　要：外源基因在动物体内、细胞内表达时,常由于各种原因导致基因沉默。目前,主要有两种策略用于打破该瓶颈:一是利用友好位点;二是优化外源基因顺式作用元件。利用友好位点就是通过定点整合技术将外源基因插入至活性转录区域;优化外源基因顺式作用元件即采用合适的启动子、增强子、内含子、染色质开放元件、核基质附着区等元件,构建出高效表达载体。对相关策略进行概述,为动物基因工程育种提供参考。The exogenous genes are often silenced in transgenic animals or cells due to a variety of reasons.Currently,there are two main strategies to break this bottleneck:one is to use safe harbors;the second is to optimize cis-acting elements of exogenous genes.Using safe harbors is to insert exogenous genes into the active transcription region via site-specific integration technologies.Optimizing cis-acting elements is to select the appropriate promoters,enhancers,introns,ubiquitous chromatin opening elements(UCOE),matrix attachment regions(MARs)to construct efficient expression vector.This review summarizes the relevant strategies for improving the expression efficiency of exogenous genes and provides a reference for genetic engineering animal breeding.

关 键 词：转基因动物 外源基因 友好位点 基因编辑 

分 类 号：Q953[生物学—动物学]