痛泻要方对肝气乘脾泄泻小鼠肠道微生物活度的影响  

Effect of Tongxieyaofang prescription on intestinal microbial activity in mice with Ganqichenpi diarrhea

作　　者：唐圆 吴仪 惠华英[1] 谭周进[1] TANG Yuan;WU Yi;HUI Huaying;TAN Zhoujin(Hu′nan University of Chinese Medicine,Changsha,Hu′nan 410208,China)

机构地区：[1]湖南中医药大学

出　　处：《中国微生态学杂志》2020年第1期17-20,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金(81874460;81703985);湖南中医药大学化学工程与技术一流学科

摘　　要：目的探讨痛泻要方对肝气乘脾泄泻小鼠肠道微生物活度的影响,为痛泻要方通过调节肠道微生物治疗肝气乘脾泄泻提供实验基础和理论依据。方法将25只SPF级雄性昆明小鼠随机分为正常组10只和模型组15只,造模成功后,随机选取正常组和模型组动物各5只,在无菌环境下提取肠道内容物和肠黏膜,剩余模型动物随机分为自然恢复组和治疗组各5只,治疗成功后处死各组动物提取肠道内容物和肠黏膜。用荧光素二乙酸法测定各样品中微生物活度。结果肝气乘脾泄泻造模小鼠肠道内容物微生物活度高于正常组,与正常组相比差异有统计学意义(P=0.01),而模型组小鼠肠黏膜微生物活度降低,与正常组相比差异有统计学意义(P=0.01)。痛泻要方治疗组和自然恢复组小鼠肠道内容物微生物活度均降低,与正常组相比差异无统计学意义(P>0.05),治疗组和自然恢复组小鼠的肠黏膜前段和后段均与正常组差异有统计学意义(P=0.001),肠黏膜中段自然恢复组与正常组差异有统计学意义(P=0.043),而治疗组与正常组没有差异。结论肝气乘脾泄泻造模使肠道内容物微生物活度增加,而使肠黏膜微生物活度降低。痛泻要方能够使肝气乘脾泄泻小鼠肠道内容物中微生物活度降低,恢复至正常水平,增加肠黏膜中段微生物活度,对肠黏膜后段微生物活度增加作用更显著。Objective To explore the effect of Tongxieyaofang prescription on intestinal microbial activity of mice with Ganqichenpi diarrhea, and provide experimental and theoretical basis for the treatment of the diarrhea with the formula by regulating intestinal microorganisms. Methods Twenty-five SPF male Kunming mice were randomly divided into the control group(n=10) or model group(n=15). After modeling, 5 mice each from the control group and model group were randomly selected to collect the intestinal contents and intestinal mucosa. The remaining model animals were randomly divided into natural recovery group or treatment group(n=5), and executed after the treatment to collect the intestinal contents and intestinal mucosa. The activity of microorganisms in each sample was determined using fluorescein diacetate method. Results The microbial activity of intestinal contents in the model group was significantly higher(P=0.01), while that of intestinal mucosa was significantly lower than in the control group(P=0.01) respectively. In the treatment group and natural recovery group, the microbial activity of intestinal contents decreased, with no significant difference from that in the control group(P>0.05);The anterior and posterior segments of intestinal mucosa in the two groups were significantly different from those in the control group(P=0.001);There was significant difference in the middle segment of intestinal mucosa between natural recovery group and control group(P=0.043), but not between treatment group and control group. Conclusion The activity of microbes in intestinal contents increased while that in intestinal mucous membrane decreased in the model mice of Ganqichenpi diarrhea. Tongxieyaofang prescription can reduce and restore the microbial activity in the intestinal contents of the model mice to normal level, increase the microbial activity in the middle and especially posterior segments of intestinal mucosa.

关 键 词：痛泻要方 肝气乘脾泄泻 肠道微生物活度 荧光素二乙酸 

分 类 号：R378[医药卫生—病原生物学]