Culturable bacterial diversity in hypolithic and peripheral soils in the west of the Hexi Corridor desert and its influencing factors  

作　　者：LiFang He ShiWeng Li GaoSen Zhang XiuKun Wu BingLin Zhang Wei Zhang 

机构地区：[1]School of Chemical and Biological Engineering,Lanzhou Jiaotong University,88 West Anning Road,Lanzhou,Gansu 730070,China [2]Key Laboratory of Extreme Environmental Microbial Resources and Engineering,Northwest Institute of Eco-Environment and Resource,Chinese Academy of Sciences,Lanzhou,Gansu 730000,China

出　　处：《Research in Cold and Arid Regions》2020年第1期47-58,共12页寒旱区科学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(31870479,31570498,and 41801045);the Key Foreign Cooperation Projects of the Bureau of International Cooperation of Chinese Academy of Sciences(131B62KYSB20160014)

摘　　要：Microbes inhabiting the desert respond sensitively to environmental changes and may be an indicator for changes in the desert ecosystem.Hypolithic microbial communities in the desert play a vital role in ecosystem processes such as soil formation and organic matter accumulation.This study investigated and compared the culturable bacterial community structure and diversity in hypolithic and peripheral soils,and the interaction between bacteria and environmental factors.The bacteria were isolated using four different kinds of media and identified by 16S rRNA gene-sequence analysis.The numbers of culturable bacteria in the hypolithic and peripheral soils ranged from 3.0×104 to 3.6×105 CFU/g and from 6.5×104 to 5.3×105 CFU/g,respectively,indicating that the bacteria number in peripheral soil was higher than that in hypolithic soil.A total of 98 species belonging to 34 genera were identified,among which Arthrobacter,Bacillus,and Streptomyces were found dominantly and widely distributed.The community of culturable bacteria had obvious sample specificity,and the diversity in hypolithic soil was higher than that in peripheral soil.On the regional scale,the distribution of culturable bacteria and the environmental factors showed regular changes.On the local scale,the high heterogeneity of the hypolithic environment determined the specificity of the number and species of culturable bacteria.Microbes inhabiting the desert respond sensitively to environmental changes and may be an indicator for changes in the desert ecosystem. Hypolithic microbial communities in the desert play a vital role in ecosystem processes such as soil formation and organic matter accumulation. This study investigated and compared the culturable bacterial community structure and diversity in hypolithic and peripheral soils, and the interaction between bacteria and environmental factors. The bacteria were isolated using four different kinds of media and identified by 16 S r RNA gene-sequence analysis. The numbers of culturable bacteria in the hypolithic and peripheral soils ranged from 3.0×104 to 3.6×105 CFU/g and from 6.5×104 to 5.3×105 CFU/g, respectively, indicating that the bacteria number in peripheral soil was higher than that in hypolithic soil. A total of 98 species belonging to 34 genera were identified, among which Arthrobacter, Bacillus, and Streptomyces were found dominantly and widely distributed. The community of culturable bacteria had obvious sample specificity, and the diversity in hypolithic soil was higher than that in peripheral soil. On the regional scale, the distribution of culturable bacteria and the environmental factors showed regular changes. On the local scale, the high heterogeneity of the hypolithic environment determined the specificity of the number and species of culturable bacteria.

关 键 词：Hexi Corridor desert hypolithic and peripheral soils culturable bacteria DIVERSITY 

分 类 号：Q938[生物学—微生物学]