低温异养硝化-好氧反硝化菌筛选及其脱氮特性  

Isolation of Cold-resistant Heterotrophic Nitrification-Aerobic Denitrification Strain and Its Nitrogen Removal Performance

作　　者：杨墨 刘乾亮 吕东伟 马军 刘惠玲 YANG Mo;LIU Qian-liang;LUDong-wei;MA Jun;LIU Hui-ling(State Key Laboratory of Urban Water Resource and Environment,School of Environment,Harbin Institute of Technology,Harbin 150090,China;Key Laboratory of Green Chemical Engineering and Technology of Heilongjiang Province College of Chemical and Environmental Engineering,Harbin University of Science and Technology,Harbin 150040,China)

机构地区：[1]哈尔滨工业大学环境学院城市水资源与水环境国家重点实验室,黑龙江哈尔滨150090 [2]哈尔滨理工大学化学与环境工程学院黑龙江省绿色化工与技术重点实验室,黑龙江哈尔滨150040

出　　处：《中国给水排水》2019年第23期100-104,共5页China Water & Wastewater

基　　金：黑龙江省博士后经费资助项目(LBH-Z17059)

摘　　要：在冬季的松花江底泥中筛选出4株高效耐低温菌,并对10℃下脱氮效率最高的33号菌进行了表型鉴定和遗传学鉴定。考察了温度、pH值和常用碳源对该菌株在低温下脱氮效果的影响,并对该菌株的低温异养硝化-好氧反硝化性能进行了研究。结果表明:该低温硝化菌株为Pseudomonas菌属,命名为Pseudomonas sp.M-33。该菌株能在2~35℃下生长并对氨氮进行去除,适宜在中性偏碱的条件下生长,能够在低温下利用柠檬酸钠、醋酸钠、丙三醇和葡萄糖等碳源。该菌株在10℃、24 h内对氨氮的去除率达到90%以上且无亚硝酸盐氮积累。M-33菌株可在好氧状态下对硝酸盐氮和亚硝酸盐氮进行反硝化去除,在硝酸盐氮反硝化过程中会产生一定的亚硝酸盐氮积累并逐渐去除。Four cold-resistant strains were isolated from the sediment of the Songhua River in winter.Phenotype analysis and genetic identification were conducted on No.33 strain which showed the highest nitrogen removal efficiency under 10℃.Effects of temperature,pH,and carbon sources on the nitrogen removal performance of No.33 strain were investigated.The performance of heterotrophic nitrification-aerobic denitrification of No.33 strain at low temperature were studied as well.The results showed that this low temperature nitrifying strain was Pseudomonas sp.,named Pseudomonas sp.M-33.M-33 strain could grow and remove ammonia nitrogen under temperature of 2-35℃,and preferred slightly alkaline environment.M-33 could utilize sodium citrate,sodium acetate,glycerol,and glucose as carbon sources at low temperature.During the heterotrophic nitrification process,M-33 removed more than 90% of ammonia nitrogen at 10℃ in 24 hours without nitrite accumulation.A certain amount of nitrite was accumulated during the denitrifying of nitrate,which was gradually degraded.

关 键 词：低温 异养硝化 好氧反硝化 脱氮特性 假单胞菌 

分 类 号：TU992[建筑科学—市政工程]