TB-LAMP联合夹层杯集菌离心涂片法快速检测对提高尘肺合并结核阳性率的研究  

Study on improving the positive rate of pneumoconiosis with tuberculosis by TB-LAMP combined with sandwich cup collecting bacteria and centrifuge smear method rapid test

作　　者：吴志伟 张秀华 闫志伟 陈娜 温陈勇 龚琴 贾爱福 郝世家 陈向东 Wu Zhiwei;Zhang Xiuhua;Yan Zhiwei;Chen Na;Wen Chenyong;Gong Qin;Jia Aifu;Hao Shijia;Chen Xiangdong(Department of Tuberculosis,Zhangjiakou Pulmonary Hospital,Zhangjiakou 075441,Hebei,China)

机构地区：[1]河北省张家口市肺科医院呼吸内科、放射科、检验科,河北张家口075441 [2]河北省张家口市疾病预防控制中心结核科,河北张家口075000

出　　处：《辽宁医学杂志》2019年第6期51-54,共4页Medical Journal of Liaoning

摘　　要：目的探讨TB-LAMP联合夹层杯集菌离心涂片法快速检测对提高尘肺合并结核阳性率的应用价值。方法选取我院在2018年1月-2019年1月就诊的尘肺患者共150例作为研究对象,用直接涂片法、BD960培养法、TB-LAMP及夹层杯集菌离心涂片法进行检测,观察对比4种检验方法结核分枝杆菌的的阳性率。结果BD960培养法、TB-LAMP及夹层杯集菌离心涂片法对比直接涂片法的阳性率结果均差异有统计学意义,P<0.05,但是前三者之间阳性率结果没有明显差异;随着尘肺期别的提高,尘肺合并结核的比例也升高,结核分枝杆菌的阳性率也升高,BD 960培养法、TB-LAMP及夹层杯集菌离心涂片法对比直接涂片法在尘肺叁期及总数上的阳性率结果均差异有统计学意义,P<0.05,但是前三者之间的阳性率结果没有统计学意义;TB-LAMP联合夹层杯集菌离心涂片法快速检测和直接涂片法的阳性率比较差异有统计学意义(P<0.01)。结论TB-LAMP联合夹层杯集菌离心涂片法快速检测能明显提高尘肺合并结核的阳性率,具有广泛的应用价值。Objective To investigate the application value of TB-LAMP combined with sandwich cup collecting bacteria and centrifuge smear method rapid test in improving the positive rate of pneumoconiosis with tuberculosis.Methods A total of 150 pneumoconiosis patients admitted to our hospital from January 2018 to January 2019 were selected as research objects,direct smear method,BD960 culture method,TB-LAMP and sandwich cup collecting bacteria and centrifuge smear method were used for detection,to observe and compare the positive rate of mycobacterium tuberculosis by 4 methods.Results The results of BD960 culture method,TB-LAMP and sandwich cup collecting bacteria and centrifuge smear method were significantly different from that of direct smear method,P<0.05,but there was no significant difference between the former three;with pneumoconiosis stage increase,the proportion of pneumoconiosis with tuberculosis also increases,the positive rate of mycobacterium tuberculosis is raised,BD960 culture method,TB-LAMP and sandwich cup collecting bacteria and centrifuge smear method compared to direct smear method in pneumoconiosis three period and the total number of positive results are significant difference,P<0.05,but there was no significant difference between the former three;the positive rate of TB-LAMP combined with sandwich cup collecting bacteria and centrifuge smear method rapid test was significantly different from that of direct smear method(P<0.01).Conclusion TB-LAMP combined with sandwich cup collecting bacteria and centrifuge smear method rapid test can significantly improve the positive rate of pneumoconiosis with tuberculosis,which is of wide application value.

关 键 词：TB-LAMP 夹层杯集菌离心涂片法 快速检测 尘肺合并结核 阳性率 

分 类 号：R135.2[医药卫生—劳动卫生] R521[医药卫生—公共卫生与预防医学]