亲本引起断乳前小鼠的位置偏爱及神经内分泌反应（英文）  

Place preference to a parent and neuroendocrine responses in preweanling mice

作　　者：马小梅 刘朝宝[1,2] 赫晨 王建礼[1,2] MA Xiao-mei;LIU Chao-bao;HE Chen;WANG Jian-li(Key Laboratory of Ecology Model and Applications of State Ethnic Affairs Commission;College of Biological Sciences and Engineering,North Minzu University,Yinchuan 750021,China)

机构地区：[1]北方民族大学国家民委生态建模与应用重点实验室,银川750021 [2]北方民族大学生物科学与工程学院,银川750021

出　　处：《生物学杂志》2020年第1期39-44,共6页Journal of Biology

基　　金：国家自然科学基金项目(31460565; 31260513);北方民族大学研究生创新项目

摘　　要：断乳前紧密的亲-子联系对于哺乳动物非常重要,这种联系促进了亲-子双方形成对彼此的情感依附,但关于幼体对亲本形成依附的机制还不清楚。精氨酸加压素(AVP)和皮质酮(CORT)在社会互作和社会联系中具有重要作用。为了探讨ICR品系小鼠幼体对母本或父本形成依附的可能机制,利用条件位置偏爱(CPP)实验检测了断乳前雄性幼体经过父鼠或母鼠4 d强化后的位置偏爱,利用免疫组织化学方法检测了形成位置偏爱时幼鼠下丘脑室旁核(PVN)和视上核(SON)AVP和c-Fos免疫活性神经元的表达,通过酶联免疫实验检测了血清CORT水平。结果发现,断乳前幼体能够产生对母鼠或父鼠信息箱的位置偏爱(P<0.05),与空箱强化相比,母鼠或父鼠强化均增加了幼体PVN的c-Fos阳性神经元表达(P<0.05),减少了血清CORT水平(P<0.05),而且经过母鼠强化的幼体PVN呈现出更多的AVP阳性神经元(P<0.05)。与父鼠强化的幼体相比,母鼠强化的幼体PVN的c-Fos阳性神经元较少(P<0.05),血清CORT水平较低(P<0.05)。SON的c-Fos和AVP免疫活性神经元没有显著性差异(P>0.05)。这些结果表明母鼠或父鼠强化可以引起幼鼠的位置偏爱,这种位置偏爱可能与PVN的神经元活动、AVP释放及血清CORT的改变有关,而且母鼠强化和父鼠强化引起幼鼠的神经内分泌活动不同。The close parent-infant bonding before weaning is critical in mammals,which facilitates the emotional attachment between each other.However,the mechanisms on filial emotional attachment towards a parent remain poorly understood.Arginine vasopressin(AVP)and corticosterone(CORT)play important roles in social interaction and social bonding.This study aimed to investigate the potential mechanisms on parent-induced attachment in ICR mice pups through conditioned place preference(CPP)test.We investigated place preference of pre-weanling male pups following four days′maternal or paternal reinforcing,while the immunoreactive(IR)neurons of hypothalamic AVP and c-Fos were detected by immunohistochemistry staining.In parallel,the levels of serum CORT were detected by enzyme-linked immunosorbent assay in mice pups.The pups conditioned with a parent showed place preference for the chamber paired with dam or sire cue with a corresponding increase in c-Fos expression in the paraventricular nucleus of the hypothalamus(PVN)and a decrease in serum CORT levels compare to the pups conditioned with empty chamber(P<0.05),and the pups conditioned with dams displayed higher levels of AVP expression in the PVN(P<0.05).In comparison with the pups conditioned with sires,the pups conditioned with dams showed lower levels of PVN c-Fos expression(P<0.05)and serum CORT concentration(P<0.05).There were not significant differences in c-Fos-and AVP-IR expression in the supraoptic nucleus of the hypothalamus(SON)(P>0.05).These results indicate the pre-weanling pups display place preference when conditioning with mothers or fathers.The neuronal activation and AVP release in the PVN and alterations of serum CORT may contribute to a parent induced-preference,and there were some differences with respect to the neuroendocrine activities of pups between maternal and paternal conditioning.

关 键 词：ICR品系小鼠 位置偏爱 精氨酸加压素 皮质酮 C-FOS 

分 类 号：Q45[生物学—生理学] Q958.12