南沙海区深海沉积物中细菌多样性分析  

Analysis on bacterial diversity in Nansha deep-sea sediments

作　　者：苏洁[1,2] 明红霞[1,2] 陈泉睿 张春鑫[1,3] 关道明 樊景凤[1,2] SU Jie;MING Hong-xia;CHEN Quan-rui;ZHANG Chun-xin;GUAN Dao-ming;FAN Jing-feng(National Marine Environmental Monitoring Center,Dalian 116023;Key Laboratory for Ecological Environmental in Coastal Areas,State Oceanic Administration,Dalian 116023;College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]国家海洋环境监测中心,大连116023 [2]国家海洋局近岸海域生态环境重点实验室,大连116023 [3]大连海洋大学水产与生命学院,大连116023

出　　处：《生物学杂志》2020年第1期50-53,共4页Journal of Biology

基　　金：国家重点研发计划“全球变化及应对专项”(2016YFA0601401);全球变化与海气相互作用专项(GASI-03-01-02-05);国家海洋局近岸海域生态环境重点实验室基金(201813)

摘　　要：为研究南沙海区深海沉积物中细菌多样性,采用16S rDNA克隆文库方法对南沙群岛3个深海站位沉积物中获得的565个阳性克隆子进行序列测定。发现3个站位所获的序列涵盖了变形菌门(Proteobacteria)、浮霉菌门(Planctomycetes)、放线菌门(Actinobacteria)、硝化螺旋菌门(Nitrospira)、绿屈挠杆菌门(Chloroflexi)、酸杆菌门(Acidobacteria)、螺旋体(Spirochaetes)、拟杆菌门(Bacteroidetes)、疣微菌门(Verrucomicrobia)和厚壁菌门(Firmicutes)共10个门。其中变形菌、浮霉菌和放线菌均为群落优势类群,这些优势类群组成与陆地以及海水中细菌优势类群存在显著差异,同时各站位细菌群落又表现出一定差异。上述结果表明南沙海区深海沉积物中具有丰富的细菌多样性,并且与其生存的环境密切相关。To study bacterial diversity in Nansha deep-sea sediments,a total of 565 clones from three Nansha deep-sea sediments were sequenced using 16S ribosomal DNA(rDNA)clone library method.The results showed that the bacterial communities from three deep-sea sites covered 10 phyla:Proteobacteria,Planctomycetes,Actinobacteria,Nitrospira,Chloroflexi,Acidobacteria,Spirochaetes,Bacteroidetes,Verrucomicrobia and Firmicutes.The predominant group in these three deep-sea sediments included Proteobacteria,Planctomycetes and Actinobacteria,which was different from the dominant group in land and marine microbiology.The above results suggested that microbial diversity of Nansha marine sediments was rich.

关 键 词：南沙 深海沉积物 细菌多样性 

分 类 号：Q938.8[生物学—微生物学]