拮抗菌诱导番茄根系分泌物鉴定及作用研究  

Identification and effect of tomato root exudates induced by antagonistic bacterium

作　　者：游薇 战鸿彬 金朝霞[1] YOU Wei;ZHAN Hong-bin;JIN Zhao-xia(School of Biological Engineering,Dalian Polytechnic University,Dalian 116033,China)

机构地区：[1]大连工业大学生物工程学院

出　　处：《生物学杂志》2020年第1期54-58,共5页Journal of Biology

基　　金：国家自然科学基金(31670604);辽宁省自然科学基金(201800645);校级创新项目(20180152090)

摘　　要：微生物防治土传病害是目前最环保有效的手段。主要采用气相色谱质谱联用(GC-MS)技术和生物测定方法,检测绿针假单胞杆菌和短小芽孢杆菌的诱导对患灰霉病番茄植株生长速度、病情指数、抗氧化酶活性以及根系分泌物种类和相对含量的影响。结果表明两种拮抗菌可以直接抑制灰霉病生长,并且两种拮抗菌的诱导使患病番茄植株生长速度未受影响,其抗氧化酶活性受到破坏程度减小。GC-MS共检测出近30种根系分泌物,拮抗菌处理组中2-酮基-D-葡萄糖酸、2-羟基丙酸、苹果酸、十八酸、亚油酸、十二酸、对苯二甲酸、十六酸甲酯、邻苯二甲酸二异辛酯组分含量明显增加。综合来说,绿针假单胞杆菌和短小芽孢杆菌不仅本身可以直接抑制灰霉菌菌体的生长,当作用到番茄植物上同样有抑制灰霉病发病的作用,并且可以通过参加植株自身代谢,以及保护植株本身抗氧化酶活性改变植物次生代谢产物的种类和含量来达到抗病的效果,有效减轻体内氧化损伤。Microbial control of soil-borne diseases is currently the most effective means of environmental protection.In this paper,GC-MS and bioassay were used to detect the growth rate,disease index,root exudates,and the effects of relative content and antioxidant enzyme activity of tomato plants induced by Pseudomonas choloeaphtis and Bacillus pumilus were studied.The results showed that two antagonists could directly inhibit the growth of Botrytis cinerea,and the induction of the two antagonists did not affect the growth rate of tomato plants,and its antioxidant enzyme activity was reduced.GC-MS detected nearly 30 root exudates.The contents of 2-keto-D-gluconic acid,2-hydroxypropionic acid,malic acid,octadecanoic acid,linoleic acid,dodecanoic acid,terephthalic acid,methyl palmitate and ortho-benzene in the Pseudomonas choloeaphtis processing group and Bacillus pumilus processing group were significantly increased.In a comprehensive way,Pseudomonas choloeaphtis and Bacillus pumilus can not only directly inhibit the growth of limestone bacteria,when the role of tomato plants also have the role of inhibiting the pathogenesis of Botrytis cinerea,and can participate in the plant's own metabolism,and to protect the antioxidant enzyme activity of the plant itself and change the species and content of the secondary metabolites of plants to achieve the effect of disease resistance,effectively reduce oxidative damage in vivo.

关 键 词：灰霉病 GC-MS 番茄 根系分泌物 抗氧化酶活性 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.412.1+3[农业科学—植物保护]