海南产和广东产沉香木中真菌的多样性分析  

Analysis of Fungi Diversity in Agarwood Wood from Hainan Province and Guangdong Province

作　　者：陈旭玉[1] 杨云[1] 刘小敏 刘杨 冯剑[1] 肖梦君 魏建和[1,2] CHEN Xu-yu;YANG Yun;LIU Xiao-min;LIU Yang;FENG Jian;XIAO Meng-jun;WEI Jian-he(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine&Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization,Hainan Branch of the Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Haikou 570311,China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education&National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所,海南省南药资源保护与开发重点实验室,国家中医药管理局沉香可持续利用重点研究室,海口570311 [2]中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室,濒危药材繁育国家工程实验室,北京100193

出　　处：《中国药学杂志》2019年第23期1933-1938,共6页Chinese Pharmaceutical Journal

基　　金：国家重点研发计划项目资助(2018YFC1706401);中组部“万人计划”项目资助(99950534);国家中药材产业技术体系资助(CARS-21);国家自然科学基金项目资助(81303312,81173481);中国医学科学院医学与健康科技创新工程资助(2016-I2M-2-003,2017-I2M-1-013)

摘　　要：目的研究海南产和广东产沉香木中真菌的组成及差异。方法利用Illumina HiSeq测序平台对沉香木中真菌基因组测序并进行生物信息学分析。结果海南省内各地区沉香木中真菌的菌落结构接近,广东省内沉香木的真菌菌落结构接近。广东产和海南产沉香木中真菌组成存在明显差异,海南产沉香木真菌多样性和丰富度高于广东产沉香木的真菌。子囊菌亚门Ascomycota为沉香木的优势门,担子菌亚门Basidiomycota次之。子囊菌亚门是海口、万宁、乐东、儋州和东莞沉香木真菌的优势种群,担子菌亚门是化州沉香木真菌的优势种群。伞菌纲Agaricomycetes为广州化州沉香木的优势种群。粪壳菌纲Sordariomycetes为东莞和儋州沉香木的优势种群。座囊菌纲Dothideomycetes为海口、乐东和万宁地区沉香木的优势种群。Pleosporales分别为东莞、海口、万宁和乐东地区沉香木真菌的优势目,Trechisporales为化州沉香木真菌的优势目,Polyporales为儋州沉香木真菌的优势目。Lignosphaeria为海口和万宁沉香木真菌的优势属,Perenniporia和Pyrigemmula分别为儋州和乐东沉香木真菌的优势属,Phaeoacremonium为化州和东莞沉香木真菌的优势属。不同地区沉香木的优势种群不同。结论海南产沉香木真菌多样性和丰富度高于广东产沉香木,不同地区沉香木真菌分布有差异。OBJECTIVE To characterize the fungal communities in agarwood wood from Hainan province and Guangdong province.METHODS Fungal ITS genes in the agarwood wood were analyzed by high-throughput pyrosequencing using Illumina HiSeq system and bioinformatically.RESULTS The fungal microbiomes exhibited much similarity among the samples from different regions in each province.And there were notable differences between Guangdong province and Hainan province.Moreover,the fungal communities in the agarwood wood from Hainan Province showed higher diversity and richness than that in the agarwood wood from Guangdong province.At the phylum level,Ascomycota most dominated in the agarwood wood,followed by Basidiomycota.Ascomycota dominated in the agarwood wood from Haikou,Wanning,Ledong,Danzhou and Dongguan regions.Basidiomycota dominated in the agarwood wood from Huazhou region.At the class level,agaricomycetes dominated in the agarwood wood from Huazhou region.Sordariomycetes dominated in the agarwood wood from Dondguan and Danzhou regions,Dothideomycetes dominated in the agarwood wood from Haikou,Ledong and Wanning regions.At the order level,Pleosporales dominated in the agarwood wood from Haikou,Ledong,Wanning and Dondguan regions.Trechisporales and Polyporales dominated in the agarwood wood from Huazhou and Danzhou regions,respectively.At the genus level,Lignosphaeria dominated in the agarwood wood from Haikou and Wanning regions,Perenniporia and Pyrigemmula dominated in the agarwood wood from Ledong and Danzhou regions,respectively. Phaeoacremonium dominated in the agarwood woodfrom Huazhou and Dondguan regions. Agarwood wood from different regions contained different dominate fungal populations. CONCLUSION The fungi in the agarwood wood from Hainan province has higher diversity and richness than that in the agawood woodfrom Guangdong province. Agarwood wood from different regions has distinct fungal communities.

关 键 词：白木香 沉香木 真菌 多样性 丰富度 

分 类 号：R282[医药卫生—中药学]