不同灭菌方法对天麻首乌片生药粉的灭菌效果及有效成分含量的影响  

Sterilizing effect of different sterilizing methods on crude powder of Tianma Shouwu tablets and their influence on the content of effective constituents

作　　者：胥双 彭艳梅 苏文俏 李跃辉 胥新元[1] XU Shuang;PENG Yanmei;SU Wenqiao;LI Yuehui;XU Xinyuan(Hunan Academy of Chinese Medicine,Chang sha 410013,Hunan,China;Hunan Provincial Center for Quality Assessment and Traceability of Traditional Chinese Medicine Preparations,Chang sha 410003,Hunan,China)

机构地区：[1]湖南省中医药研究院,湖南长沙410013 [2]湖南省传统中药制剂质量评价和溯源工程中心,湖南长沙410003

出　　处：《湖南中医杂志》2019年第12期115-116,146,共3页Hunan Journal of Traditional Chinese Medicine

摘　　要：目的:考察不同灭菌方法用于天麻首乌片原生药粉的灭菌效果及灭菌对目标成分天麻素、阿魏酸及二苯乙烯苷含量的影响。方法:分别采用湿热、微波、75%乙醇流通蒸汽、60Co辐照4种灭菌方法对天麻首乌片中原生药粉进行灭菌处理,测定灭菌前后原生药粉的微生物限度;采用高效液相色谱法测定灭菌前后天麻素、阿魏酸和二苯乙烯苷的含量。结果:微生物限度检测结果显示,上述4种灭菌方法的灭菌率依次分别为99.21%、95.24%、77.78%、99.21%;目标成分检测结果表明,60Co辐照灭菌对天麻素、阿魏酸和二苯乙烯苷含量影响最小,其次是75%乙醇流通蒸汽灭菌,而微波和湿热2种灭菌方法对天麻素、阿魏酸和二苯乙烯苷含量的影响最大。结论:综合灭菌效果及对有效成分的影响,天麻首乌片原生药粉灭菌方法建议选用60Co辐照灭菌。Objective:To investigate the sterilizing effect of different sterilizing methods on crude powder of Tianma Shouwu tablets and the influence of sterilization on the content of gastrodin,ferulic acid,and stilbene glucoside.Methods:The crude powder of Tianma Shouwu tablets was sterilized by moist heat sterilization,microwave,75%alcohol circulating steam,and 60Co irradiation,respectively,and microbial limit was measured before and after sterilization.High-performance liquid chromatography was used to measure the content of gastrodin,ferulic acid,and stilbene glucoside before and after sterilization.Results:The results of microbial limit showed that the above four methods had a sterilization rate of 99.21%,95.24%,77.78%,and 99.21%,respectively.As for the content of the above constituents,60Co irradiation had the least influence on the content of gastrodin,ferulic acid,and stilbene glucoside,followed by 75%alcohol circulating steam,and microwave and moist heat sterilization had the greatest influence on the content of these constituents.Conclusion:Based on comprehensive sterilizing effect and the influence on the content of effective constituents,60Co irradiation is suggested as the preferred sterilizing method for crude powder of Tianma Shouwu tablets.

关 键 词：天麻首乌片 灭菌 湿热 75%乙醇流通蒸汽 微波 60Co辐照 实验研究 

分 类 号：R282.4[医药卫生—中药学]