Toll样受体9基因多态性和幽门螺杆菌感染的关系  

Associations of TLR9 gene polymorphisms with Helicobacter pylori infection

作　　者：卫星如 马立聪 田旭阳[2] 党彤[2] 高芳[3] 贾彦彬[1,2,4] Wei Xingru;Ma Licong;Tian Xuyang(School of Basic Medicine and Forensic Medicine,Baotou Medical College,Baotou 014060;Inner Mongolia Institute of Gastroenterology,The Second Affiliated Hospital of Baotou Medical College,Baotou 014030)

机构地区：[1]包头医学院基础医学与法医学院,包头014060 [2]包头医学院第二附属医院内蒙古消化病研究所,包头014030 [3]包头医学院医学技术学院,包头014060 [4]包头医学院护理学院,包头014060

出　　处：《安徽医科大学学报》2020年第1期142-145,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30960169、 81250024、81650017);内蒙古自然科学基金[编号:2016MS0805、2017MS(LH)0807];包头医学院科学研究基金(编号:BYJJ-DF 201603)

摘　　要：目的探讨Toll样受体9(TLR9)基因多态性与幽门螺杆菌感染的关系。方法选取体检者683例的血液样本,采用酶联免疫吸附测定方法(ELISA)将样本分为幽门螺杆菌阴性组(n=305)和幽门螺杆菌阳性组(n=378),采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法对TLR9 rs187084、rs352140、rs164640位点进行基因分型。结果TLR9 rs187084 TC基因型可使幽门螺杆菌感染风险降低(TC vs TT:OR=0.520,95%CI=0.329~0.820);rs164640携带AG+GG基因型者幽门螺杆菌感染风险增加(AG+GG vs AA:OR=1.569,95%CI=1.126~2.187);rs352140与幽门螺杆菌感染没有关联。结论TLR9 rs187084、rs164640可能在幽门螺杆菌的感染中起到了一定的作用。Objective To investigate the relationship between Toll-like receptor 9(TLR9)gene polymorphisms and Helicobacter pylori(H.pylori)infection.Methods A total of 683 blood samples were collected.The samples were divided into H.pylori negative group(n=305)and H.pylori positive group(n=378)by enzyme-linked immunosorbent assay(ELISA)method.Results TLR9 rs187084,rs352140 and rs164640 were genotyped by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).TLR9 rs187084 TC genotype reduced the risk of H.pylori infection(TC vs.TT:OR=0.520,95%CI=0.329~0.820).The risk of H.pylori infection increased in rs164640 AG+GG genotype carriers(AG+GG vs.AA:OR=1.569,95%CI=1.126~2.187).rs352140 was not associated with the risk of H.pylori infection.Conclusion TLR9 rs187084 and rs164640 might play a role in H.pylori infection.

关 键 词：幽门螺杆菌 TLR9 单核苷酸多态性 

分 类 号：R34[医药卫生—基础医学]