葛根素对沙鼠脑缺血再灌注损伤的保护作用及其机制研究  

Protective effect and mechanism of puerarin on cerebral ischemia-reperfusion injury in gerbils

作　　者：夏杨[1] 贾志姣 文姝[3] 李若文 XIA Yang;JIA Zhi-jiao;WEN Shu;LI Ruo-wen(Fourth Department of Internal Medicine,Beijing Municipality Hospital of PAP,Beijing 100027,China)

机构地区：[1]武警北京总队医院内四科,北京100027 [2]北京中医药大学东方医院耳鼻喉科,北京100078 [3]武警北京总队医院护理部,北京100027 [4]武警北京总队医院保健科,北京100027

出　　处：《武警后勤学院学报（医学版）》2019年第8期6-10,共5页Journal of Logistics University of PAP（Medical Sciences）

基　　金：武警北京总队医院自主选题项目(2017-XY-96)

摘　　要：【目的】探讨葛根素预处理对脑缺血再灌注损伤(cerebral ischemia/reperfusion injury,CI/RI)沙鼠脑组织炎症反应的影响。【方法】将50只沙鼠随机分为葛根素3个剂量组(剂量分别为50、25、12.5 mg/kg)、模型组、假手术组,模型制备前3 d各组开始腹腔注射给药,采用双侧颈总动脉夹闭术制备CI/RI模型。采用卒中指数评估再灌注6 h内的神经症状,采用免疫组化法检测再灌注6 h后脑海马组织核因子-κB(nuclear factor-κB,NF-κB)、细胞间黏附分子1(intercellular cell adhesion molecule-1,ICAM-1)和基质金属蛋白酶9(matrix metalloproteinases-9,MMP-9)的表达。【结果】与模型组相比,葛根素高、中剂量预处理CI/RI沙鼠的卒中指数评分均有明显降低(P<0.01);与模型组相比,葛根素各剂量预处理均可明显下调脑海马组织NF-κB和ICAM-1的阳性表达,组间差异有统计学意义(P<0.05或P<0.01);葛根素高、中剂量脑海马组织MMP-9的表达有明显降低,与模型组相比差异有统计学意义(P<0.05)。【结论】葛根素预处理对沙鼠CI/RI具有脑保护作用,其作用机制可能是由于葛根素可抑制炎症因子表达,缓解脑组织的炎症反应。【Objective】To explore the effect of puerarin(PUE)pretreatment on inflammatory reaction of brain tissue in gerbils with cerebral ischemia-reperfusion injury(CI/RI).【Methods】A total of 50 gerbils were randomly divided into PUE high,medium and low dose groups(50,25 and 12.5 mg/kg,respectively),sham and model groups.PUE was intraperitoneally injected for 3 days before the model preparation.CI/RI model in gerbil was achieved by bilateral common carotid occlusion followed by 6-hour reperfusion.Stroke index was used to evaluate neurological symptoms of gerbils during 6-hour reperfusion.The activities of nuclear factor-κB(NF-κB),intercellular cell adhesion molecule-1(ICAM-1)and matrix metalloproteinases-9(MMP-9)in hippocampal tissue of CI/RI gerbils were examined by immunohistochemistry after 6-hour reperfusion.【Results】Compared with the model group,stroke index in PUE pretreatment groups(50 and 25 mg/kg)was markedly improved(P<0.01).Compared with the model group,the expression of NF-κB and ICAM-1 in hippocampi tissue in PUE pretreatment groups were significantly reduced(P<0.05 or P<0.01).PUE(50 and 25 mg/kg)markedly inhibited MMP-9 expression in hippocampal tissue compared with the model group(P<0.05).【Conclusion】PUE pretreatment can protect the brain tissue of CI/RI gerbils.Its action may be related with the inhibition of inflammatory factors and relieving inflammation in brain tissue.

关 键 词：葛根素 脑缺血再灌注损伤 核因子-ΚB 细胞间黏附分子1 基质金属蛋白酶9 

分 类 号：R285.5[医药卫生—中药学]