艾灸对胃荷瘤大鼠瘤体增长的抑制作用  

Inhibitory Effect of Moxibustion on Tumor Growth in Rats with Gastric Tumor Bearing

作　　者：谭静[1] 阳仁达[1] 赵欢 彭卓隽 欧阳里知 林亚平[1] Tan Jing;Yang Renda;Zhao Huan;Peng Zhuojun;Ouyang Lizhi;Lin Yaping(Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿学院

出　　处：《世界科学技术-中医药现代化》2019年第9期1994-2000,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会面上项目（81574077）：艾灸诱导HSP-PCs产生并回输体内对荷瘤大鼠肿瘤细胞抑制效应的研究，负责人：林亚平;湖南省教育厅优秀青年项目（18B257）：从促炎因子→NF-κB→UPS途径，探讨艾灸对胃癌恶病质大鼠骨骼肌消耗的治疗作用与机理，负责人：谭静

摘　　要：目的观察艾灸对胃荷瘤大鼠瘤体增长的抑制作用。方法50只健康SD大鼠,随机分为空白组、假手术组、模型组、艾灸组、红外组5组,每组10只。采用Walker-256细胞实体瘤组织移植胃部制备动物模型。成模后,艾灸组、红外组分别施以温和灸、红外线治疗20 min,每日1次,持续21 d。实验期间,观察大鼠的生存状态,记录饮食、体重等变化。治疗结束后,摘取胃部,测量肿瘤体积、计算生长抑制率。采用免疫组化法,观测肿瘤细胞增殖细胞核抗原(Proliferating Cell Nuclear Antigen,PCNA)的表达,TUNEL观测肿瘤细胞凋亡。结果实验期间,模型组动物生存状态持续变差,成模5 d后生存状态积分明显低于空白组(P<0.01);艾灸组动物生存状态自治疗10 d后开始改善,治疗15 d后积分明显高于模型组(P<0.01)。治疗结束后,与模型组比较,艾灸组瘤体增长抑制率达41.89%,瘤体内出现大量坏死灶,血管较少,肿瘤细胞增殖减少、凋亡增多(P<0.01)。与红外组比较,艾灸组在改善生存状态、抑制肿瘤体积增长、减少肿瘤细胞增殖及转移、促进肿瘤细胞凋亡上作用更明显(P<0.05)。结论艾灸可明显改善荷瘤大鼠的生存状态,通过减少肿瘤细胞的增殖、促进肿瘤细胞的凋亡以抑制瘤体的增长。Objective To observe the inhibitory effect of moxibustion on tumor growth in gastric tumor bearing rats.Methods Fifty healthy SD rats were randomly divided into 5 groups:blank group,sham operation group,model group,moxibustion group and infrared group,with 10 rats in each group.Walker-256 cell solid tumor tissue was transplanted into the stomach to prepare animal models.After modeling,moxibustion group and infrared group were treated with mild moxibustion and infrared ray for 20 minutes,once a day for 21 days.During the experiment,the survival state of rats was observed and recorded.After treatment,the stomach was removed,the tumor volume was measured and the growth inhibition rate was calculated.The expression of proliferating cell nuclear antigen(PCNA)and apoptosis of tumor cells were observed by immunohistochemistry.Results During the experiment,the survival state of the model group was worse than that of the blank group.After treatment,compared with the model group,the inhibition rate of tumor growth in moxibustion group was 41.89%,there were a lot of necrotic foci,fewer blood vessels in the tomor and the proliferation of the tumor cell decreased while the apoptosis increased(P<0.01).Compared with infrared group,moxibustion group had more obvious effects on improving survival state,inhibiting tumor volume growth,reducing tumor cell proliferation and metastasis and promoting tumor cell apoptosis(P<0.05).Conclusion Moxibustion can obviously improve the survival state of gastric tumor-bearing rats and also can inhibit the growth of tumor by reducing the proliferation,promoting the apoptosis of tumor cells.

关 键 词：艾灸 红外线 胃癌 增殖 凋亡 

分 类 号：R245.81[医药卫生—针灸推拿学]