基于超高效液相色谱指纹图谱和主成分分析法评价不同主产地半枝莲的质量  

Study on Quality of Scutellaria barbata D.Don in Different Main Producing Areas Based on UPLC Fingerprint and Principal Component Analysis

作　　者：杨敏娟 罗宇琴 官永河 朱德全 Yang Minjuan;Luo Yuqin;Guan Yonghe;Zhu Dequan(Guangdong Yifang Pharmaceutical Co.,Letd.,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司

出　　处：《世界科学技术-中医药现代化》2019年第10期2247-2252,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广东一方制药有限公司佛山市重大科技项目应用型核心技术攻关领域（2016AG100912）：中药配方颗粒国家标准研究，负责人：魏梅

摘　　要：目的建立半枝莲药材的超高效液相色谱(UPLC)指纹图谱,综合比较不同主产区的半枝莲质量;方法采用UPLC建立半枝莲药材的指纹图谱,应用超高效液相飞行时间高分辨质谱技术对色谱峰进行指认;应用PCA法对UPLC指纹图谱进行分析。结果半枝莲药材UPLC指纹图谱共11个共有峰,其中4个为野黄芩苷、芹菜素-7-O-葡萄糖苷、野黄芩素、芹菜素;产自河南、安徽和四川3个产地的半枝莲药材相似度为0.871-0.999;从主成分分析来看,野黄芩苷、芹菜素-7-O-葡糖糖苷、成分1、4、6、7、8及10在半枝莲质量上起重大作用。结论UPLC指纹图谱结合PCA可以客观地评价不同主产地半枝莲药材的质量及各成分对质量的贡献。Objective To evaluate the quality of Scutellaria barbata D.Don in different main producing areas.Methods An Ultra Performance Liquid Chromatograph(UPLC)method was developed to establish the fingerprint of Scutellaria barbata D.Don.UPLC/High Resolution Mass Spectrometry were applied to identify common peaks,and PCA was used to analyze UPLC fingerprints.Results There were 11 common peaks in the UPLC fingerprint of all Scutellaria barbata D.Don,and the 4 peaks were scutellarin,apigenin-7-glucoside,scutellarein,apigenin.The similarity of Scutellaria barbata D.Don from Henan,Anhui and Sichuan was 0.871-0.999.According to PCA,scutellarin,apigenin-7-Oglucoside,components 1,4,6,7,8 and 10 played an important role in the quality of Scutellaria barbata D.Don.Conclusion The UPLC fingerprint combined with PCA can objectively evaluate the quality of Scutellaria barbata D.Don in different main producing areas,and the contribution of each component to quality.

关 键 词：半枝莲 超高效液相色谱 指纹图谱 超高效液相飞行时间高分辨质谱技术 主成分分析 

分 类 号：R282.5[医药卫生—中药学]