利用Loxp/Cre重组酶体系构建脂肪组织GGPPS特异性缺失的小鼠模型  

Constructing adipose tissue GGPPS-specific knockdown mice by Loxp/Cre recombinant enzyme system

作　　者：薛晓萌 刘健 XUE Xiaomeng;LIU Jian(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China)

机构地区：[1]合肥工业大学食品与生物工程学院

出　　处：《合肥工业大学学报（自然科学版）》2020年第2期270-273,共4页Journal of Hefei University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(31671485)

摘　　要：香叶草基合成酶(Geranylgeranyl bisphosphate synthase,GGPPS)与囊泡运输过程中小G蛋白的翻译后修饰密切相关。文章选择同时具有2个Loxp位点基因和GGPPS基因的Ggpps-floxed小鼠与带有脂肪组织特异性启动子aP2的Cre小鼠aP2-Cre进行杂交,以获得脂肪组织GGPPS特异性缺失的小鼠Ad Ggpps KO。经过基因鉴定和多组织的定量聚合酶链反应(polymerase chain reaction,PCR)以及Western blot确认,得到的小鼠模型的脂肪组织GGPPS缺失。Geranylgeranyl bisphosphate synthase(GGPPS) was closely related with vesicle trafficking and post-translational modifications of small G proteins. Ggpps-floxed mice with two Loxp genes and GGPPS gene and aP2-Cre mice with adipose tissue specific promoter aP2 were selected to generate adipose tissue GGPPS-specific knockdown mice Ad Ggpps KO. The results of genotyping, quantitative polymerase chain reaction(PCR) and Western blot showed that the expression of GGPPS in adipose tissue was reduced.

关 键 词：Loxp/Cre 香叶草基合成酶(GGPPS) 脂肪组织 小鼠 

分 类 号：Q786[生物学—分子生物学]