大围山微型鸡与科宝肉鸡免疫球蛋白含量及CATH-2基因表达差异研究  

Study on Immunoglobulin Content and CATH-2 Gene Expression in Daweishan Mini Chicken and Cobb Broiler

作　　者：赵净颖 闫世雄 王秋婷 李涛 豆腾飞 段小花 李琦华[1,3] 葛长荣[1] 贾俊静[1,3] ZHAO Jingying;YAN Shixiong;WANG Qiuting;LI Tao;DOU Tengfei;DUAN Xiaohua;LI Qihua;GE Changrong;JIA Junjing(College of Animal Science and Technology,Yunnan Agricultural University,Kunming,Yunnan 650201;Yunnan University of Chinese Medicine,Kunming,Yunnan 650201;Key Laboratory of Animal Nutrition and Feed,Yunnan Agricultural University,Kunming,Yunnan 650201)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南中医药大学,云南昆明650201 [3]云南农业大学动物营养与饲料重点实验室,云南昆明650201

出　　处：《中国家禽》2020年第1期12-16,共5页China Poultry

基　　金：国家自然科学基金项目(31560618、U1702232、31572359);云南省自然科学基金重点项目(2016FA046);云南省科技厅南希·莱恩院士工作站(2017IC048);云岭学者及云岭产业技术领军人才培养经费。

摘　　要：试验以相同条件下饲养的4、8、12周龄的大围山微型鸡和科宝肉鸡为研究对象,分别测定其免疫球蛋白含量(IgA、IgG、IgM)及胸肌、肝脏、小肠中的CATH-2 mRNA表达量,比较二者之间的差异。结果显示:大围山微型鸡血液中免疫球蛋白含量总体上低于科宝肉鸡,两个鸡种随着日龄的增加IgA、IgG含量均呈现先下降后上升趋势,IgM含量呈现下降趋势,且两个试验鸡种在4周龄时IgA、IgM、IgG含量均不同程度的高于8、12周龄。CATH-2 mRNA表达量在两个鸡种间存在差异,大围山微型鸡胸肌和肝脏中CATH-2 mRNA表达量均低于科宝肉鸡,而小肠中CATH-2 mRNA表达量显著高于科宝肉鸡。结果表明:在相同饲养条件下,大围山微型鸡免疫机能强于科宝肉鸡,具有潜在的较强的抗感染能力。This study measured the contents of immunoglobulin(IgA, IgG, IgM) and CATH-2 gene mRNA expression in the breast muscle, liver and small intestine of Daweishan mini chickens and Cobb broilers at the age of 4, 8 and12 weeks under the same feeding conditions to compare the differences between the two breeds. The results showed that the blood immunoglobulin content of Daweishan mini chickens was lower than that of Cobb broilers. The IgA and IgG contents of the two breeds decreased firstly and then increased with the increase of age, and IgM content showed a downward trend. The contents of IgA, IgM and IgG of the two breeds at 4 weeks of age were all higher than those of 8 and 12 weeks of age.The CATH-2 gene mRNA expression was different between the two breeds. The CATH-2 gene mRNA expressions in Daweishan mini chickens′ breast muscle and liver were lower than Cobb broilers, while the CATH-2 gene m RNA expression in small intestine was significantly higher than Cobb broilers. The results indicated that the immune function of Daweishan mini chickens was stronger than Cobb broilers, and Daweishan mini chickens had potential and stronger ability to resist infection under the same feeding conditions.

关 键 词：免疫性状 血液免疫球蛋白 CATH-2基因 大围山微型鸡 科宝肉鸡 

分 类 号：S831.2[农业科学—畜牧学]