原发性干燥综合征患者高迁移率族蛋白B1与辅助性T细胞17/调节性T细胞平衡的关系  

Relationship between high mobility group protein B1 level and helper T cell 17/regulatory T cell balance in patients with primary Sj?gren’s syndrome

作　　者：李雪 程婷 任效瑛 王娟 侯子琪 梁树芬[2] LI Xue;CHENG Ting;REN Xiaoying;WANG Juan;HOU Ziqi;LIANG Shufen(The Second Clinical Medical College,Shanxi Medical University,Shanxi Province,Taiyuan030001,China;Department of Clinical Laboratory,the Second Hospital of Shanxi Medical University,Shanxi Province,Taiyuan030000,China)

机构地区：[1]山西医科大学第二临床医学院,山西太原030001 [2]山西医科大学第二医院检验科,山西太原030000

出　　处：《中国医药导报》2020年第4期167-170,共4页China Medical Herald

基　　金：山西省软科学研究项目（2017041038-3）

摘　　要：目的探讨原发性干燥综合征(pSS)患者外周血中高迁移率族蛋白B1(HMGB1)水平与辅助性T细胞17(Th17)/调节性T细胞(Treg)平衡的关系。方法选取2018年5月~2019年5月山西医科大学第二医院风湿免疫科初诊pSS患者40例为pSS组,同期健康体检者20名为对照组。采集肘静脉血,流式细胞仪检测Th17、Treg细胞个数,计算Th17/Treg比值;流式细胞仪微球捕获芯片技术检测血清白细胞介素(IL)-17、IL-6、IL-10水平;酶联免疫吸附测定法检测HMGB1、转化生长因子-β(TGF-β)水平。将上述各指标进行组间比较,并分析HMGB1与上述细胞及相关细胞因子的相关性。结果pSS组HMGB1、Th17、Th17/Treg水平高于对照组,而Treg水平低于对照组,差异有统计学意义(P<0.05)。pSS组IL-17、IL-6、TGF-β水平高于对照组,而IL-10水平低于对照组,差异有统计学意义(P<0.05)。相关性分析表明HMGB1与Th17、Th17/Treg、IL-17、IL-6水平呈正相关(r=0.738、0.502、0.762、0.578,均P<0.05),与Treg、IL-10水平呈负相关(r=-0.657、-0.686,均P<0.05),与TGF-β水平无明显相关性(P>0.05)。结论pSS患者外周血HMGB1异常高表达,且与健康人相比存在明显的T细胞免疫异常,提示HMGB1可能通过调节Th17/Treg平衡参与pSS的发生、发展。Objective To investigate the relationship between the level of high mobility group box 1(HMGB1)and the balance of helper T cell 17(Th17)/regulatory T cells(Treg)in peripheral blood of patients with primary Sjogren′s syndrome(pSS).Methods Forty patients with pSS in Rheumatology Department of the Second Hospital of Shanxi Medical University from May 2018 to May 2019 were selected as pSS group,and 20 healthy subjects were control group.Elbow venous blood was collected.The number of Th17 and Treg cells was detected by flow cytometry,and Th17/Treg ratio was calculated.Cytometric bead array was used to detect serum interleukin(IL)-17,IL-6 and IL-10 levels.Enzyme-linked immunosorbent was used to detect the levels of HMGB1 and transforming growth factor-β(TGF-β).Each of the above indicators was compared between two groups,and the correlation between HMGB1 and the expression of various cells and cytokines was analyzed.Results The levels of HMGB1,Th17 and Th17/Treg in pSS group were higher than those in control group,while Treg was lower than that in control group,and the difference was statistically significant(P<0.05).The levels of IL-17,IL-6 and TGF-βin pSS group were higher than those in control group,while IL-10 was lower than that in control group,the differences were statistically significant(P<0.05).Correlation analysis showed that HMGB1 was positively correlated with Th17,Th17/Treg,IL-17 and IL-6 levels(r=0.738,0.502,0.762,0.578,all P<0.05).There was a negative correlation between the levels of HMGB1 and Treg,IL-10(r=-0.657,-0.686,all P<0.05),no significant correlation with TGF-β(P>0.05).Conclusion HMGB1 in peripheral blood of patients with pSS is abnormally high,and there is obvious T cell immune abnormality compared with healthy people,suggesting that HMGB1 may participate in the development of pSS by regulating Th17/Treg balance.

关 键 词：原发性干燥综合征 高迁移率族蛋白B1 辅助性T细胞17 调节性T细胞 

分 类 号：R593[医药卫生—内科学]