依达拉奉联合缺血后处理对大鼠脑缺血再灌注损伤的影响  

Effect of Edaravone combined with ischemic postconditioning on cerebral ischemia-reperfusion injury in rats

作　　者：王颖[1] 周红群 殷梅[1] 缪薇[1] 朱榆红[1] WANG Ying;ZHOU Hongqun;YIN Mei;MIAO Wei;ZHU Yuhong(Department of Neurology,the Second Affiliated Hospital of Kunming Medical University,Yunnan Province,Kunming 650031,China)

机构地区：[1]昆明医科大学第二附属医院神经内科

出　　处：《中国医药导报》2020年第5期13-17,F0004,共6页China Medical Herald

基　　金：云南省医疗卫生单位内设研究机构科研项目（2016NS263）

摘　　要：目的探讨依达拉奉(Eda)与缺血后处理(IP)联合使用后对脑缺血再灌注损伤(I/R)的影响,并与单独应用IP比较,评价Eda联合IP是否能对I/R产生叠加保护作用。方法选择SD大鼠36只,根据随机数字表法将其分为对照组(I/R组)、IP组、IP+Eda组,每组12只。I/R组采用线栓法建立大鼠I/R模型,IP组采用线栓法建立大鼠I/R模型的同时给予IP干预,IP+Eda组采用线栓法建立大鼠I/R模型的同时给予Eda和IP干预。术后48 h对大鼠进行神经功能缺损评分、脑梗死体积测定;采用荧光定量PCR、Western blot、免疫组化法检测大鼠脑组织中血管紧张素Ⅱ1型和2型受体(AT1、AT2)的表达;检测各组大鼠超氧化物歧化酶(SOD)抑制率。结果与I/R组比较,IP+Eda组和IP组神经功能缺损评分降低,大鼠脑梗死体积缩小,AT1、AT2的mRNA和蛋白相对表达量降低,AT1、AT2阳性细胞数减少,SOD抑制率升高,差异有统计学意义(P<0.01或P<0.05)。IP+Eda组与IP组比较,上述指标差异均无统计学意义(P>0.05)。结论IP+Eda组能对大鼠I/R产生保护作用,但两者联合后并不会产生叠加的保护效应。Objective To investigate the effect of Edaravone(Eda)combined with ischemic postconditioning(IP)on cerebral ischemia-reperfusion injury(I/R),and to evaluate whether Eda combined with IP have superimpose protective effects on I/R compared with IP alone.Methods Thirty-six SD rats were selected and divided into the control group(I/R group),IP group and IP+Eda group according to the random number table method,with 12 rats in each group.The rat I/R model was established in the I/R group by suture method.The rat I/R model was established by suture method in the IP group with IP intervention.The rat I/R model was established by suture method in the IP+Eda group with Eda and IP intervention.At 48 h after the operation,the rats were evaluated for neurological deficits and the volume of cerebral infarction was measured.By fluorescence quantitative PCR,Western blot and immunohistochemical method the expression of tissue angiotensinⅡwith type 1 and type 2 receptor(AT1,AT2)in the rat brain tissue was detected.The inhibition rate of superoxide dismutase(SOD)of rats in each group was detected.Results Compared with I/R group,IP+Eda group and IP group showed a decrease in neurological deficit score,a decrease in cerebral infarction volume,a decrease in mRNA and protein expression of AT1 and AT2,a decrease in the number of AT1 and AT2 positive cells,and an increase in SOD inhibition rate,with statistically significant differences(P<0.01 or P<0.05).There was no significant difference in the above indicators between the IP+Eda group and the IP group(P>0.05).Conclusion The IP+Eda group can protect rat I/R,but the combination of the two groups does not produce a superimposed protective effect.

关 键 词：脑缺血/再灌注损伤 依达拉奉 缺血后处理 血管紧张素Ⅱ1型受体 血管紧张素Ⅱ2型受体 

分 类 号：R54[医药卫生—心血管疾病]