不同LED光色对家兔繁殖性能的影响及其分子调控机制  

Effects of different LED light colors on reproductive performance of rabbits and their molecular regulation mechanism

作　　者：潘孝青[1,2] 王杏龙[1] 杨杰[2] 秦枫[2] 邵乐[2] 李健[2] 张霞[2] 翟频[2] PAN Xiao-qing;WANG Xing-long;YANG Jie;QIN Feng;SHAO Le;LI Jian;ZHANG Xia;ZHAI Pin(College of Animal Science and Technology,Yangzhou University,Yangzhou 212400,China;Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州212400 [2]江苏省农业科学院畜牧研究所,江苏南京210014

出　　处：《江苏农业学报》2020年第1期136-146,共11页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科学院探索性颠覆性创新计划项目[(17)2012];国家兔产业技术体系-南京综合试验站项目(CARS-43-G-2);国家科技基础条件平台-特种经济动物种质资源平台项目(TZDWZYPT2019-02)

摘　　要：为探讨不同LED光色影响母兔繁殖效率的分子机制,将单笼饲养的300只后备母兔随机分成5组进行不同LED光色处理,每组3个重复,每个重复20只。在4个不同LED光色处理组兔笼上方60 cm处向下分别投射LED红光(660 nm)、LED绿光(540 nm)、LED蓝光(480 nm)和LED白光(400~760 nm),对照组投射白炽灯光(400~1050 nm)。母兔每周接受3次补光(LED光处理),即每周的周五至周日下午18∶00至次日上午6∶00,时长为12 h,预试验7 d,正试期90 d。LED灯及白炽灯的光照度均设置为100 lx。结果显示:LED红光组母兔同期发情率与白炽灯光对照组及LED白光组相比,分别提高20.89个百分点和10.51个百分点,差异显著(P<0.05);与绿光组及蓝光组相比,分别提高4.66个百分点和7.33个百分点,差异不显著(P>0.05)。LED红光组母兔卵巢红色大卵泡数量有增加趋势,但各试验组间卵巢红色大卵泡数量差异不显著(P>0.05)。与白炽灯光、LED白光、绿光、蓝光相比,LED红光能促进母兔垂体GnRHR mRNA表达,但差异不显著(P>0.05);与LED白光、绿光、蓝光相比,LED红光显著提高了卵巢组织中FSHR、LHR基因mRNA的表达(P<0.05);与白炽灯光对照相比,LED红光有提高卵巢组织中FSHR、LHR基因mRNA表达水平的趋势,但差异不显著(P>0.05)。在原始卵泡(PrF)阶段与初级卵泡(PF)阶段,不同LED光色处理的卵泡数量差异不显著(P>0.05);与原始卵泡(PrF)及初级卵泡(PF)相比,由于卵泡逐级闭锁,次级卵泡(SF)数量显著降低,不同组间开始出现差异,与LED绿光组及LED蓝光组相比,LED红光组、LED白光组及白炽灯光对照组次级卵泡(SF)数量显著增加(P<0.05);由于卵泡进一步闭锁,与原始卵泡(PrF)、初级卵泡(PF)以及次级卵泡(SF)相比,三级卵泡(TF)数量显著减少,不同组间三级卵泡(TF)数量也呈现不同差异,与LED绿光组、LED蓝光组及白炽灯光对照组相比,LED红光组三级卵泡(TF)数量显著增加(P<0.05),但与LED白光组�To explore the molecular mechanism of different LED light colors affecting the reproductive efficiency of female rabbits,three hundred female rabbits reared in a single cage were randomly divided into five groups for different LED light colors treatments.LED red light(660 nm),LED green light(540 nm),LED blue light(480 nm)and LED white light(400-760 nm)were projected downwards at 60 cm above the rabbit cage.The control group was incandescent lamp(400-1050 nm).Female rabbits received the treatment with supplementary light three times a week,the duration was 12 hours(12D∶12L),the pre-experiment was seven days,and the standard test was 90 days.The light intensity of LED lamp and incandescent lamp was set as 100 lx.Compared with incandescent lamp group and LED white light group,the estrus rate of female rabbits in LED red light group increased by 20.89 percentage points and 10.51 percentage points(P<0.05).Compared with LED green light group and LED blue light group,the estrus rate of female rabbits in LED red light group increased by 4.66 percentage points and 7.33 percentage points(P>0.05).The number of large red follicles in the ovaries of the female rabbits in the LED red light group increased,but there was no significant difference between the experimental groups(P>0.05).Compared with incondescent light,LED white light,LED green light and LED blue light,LED red light could promote the mRNA expression of GnRHR in the pituitary gland of female rabbits,but the difference was not significant(P>0.05).Compared with LED white light,LED green light and LED blue light,LED red light significantly increased the expression of FSHR and LHR in ovarian tissue(P<0.05).Compared with the incandescent light,LED red light could increase mRNA expression of FSHR and LHR gene in ovarian tissue,but the difference was not significant(P>0.05).In the primordial follicles(PrF)stage and the primary follicles(PF)stage,the effect of different LED light colors on the number of follicles was not significant(P>0.05).Compared with PrF and PF,the

关 键 词：LED光 兔 繁殖性能 分子调控 

分 类 号：S829.146[农业科学—畜牧学]