棘孢木霉T-6对烟草促生及对黑胫病和根黑腐病的抗病作用  

Effects of Trichoderma asperellum T-6 on Growth Promotion, Control of Black Shank and Black Root Rot in Tobacco

作　　者：宋玉娟 韩雪莲 张安盛[2] 王玉军[1] 高克祥[1] 梁元存[1] SONG Yujuan;HAN Xuelian;ZHANG Ansheng;WANG Yujun;GAO Kexiang;LIANG Yuancun(College of Plant Protection,Shandong Agricultural University,Tai’an,Shandong 271018,China;Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan,250100,China)

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]山东省农业科学院植保所,济南250100

出　　处：《中国烟草科学》2020年第1期50-55,共6页Chinese Tobacco Science

基　　金：山东省现代农业烟草体系烟草病虫防控岗位（SDAIT-25-3）

摘　　要：利用生防菌防治土传病害是植物病害防治中的重要措施,在温室盆栽条件下研究了棘孢木霉T-6对烟草促生和抗病效果。结果表明,棘孢木霉T-6对烟草黑胫病菌、烟草根黑腐病菌的菌丝生长抑制率分别达49.62%、59.23%;温室盆栽条件下,棘孢木霉T-6对黑胫病和根黑腐病的防治效果分别达到74.45%和75.14%。棘孢木霉T-6处理后提高了烟草叶片叶绿素含量、类胡萝卜素含量和超氧化物歧化酶、过氧化物酶、过氧化氢酶的活性,棘孢木霉T-6处理的烟株生长状况明显好于对照。棘孢木霉T-6能够促进烟株生长和提高烟草对黑胫病和根黑腐病的抗性,具有良好的应用潜力。Using Trichoderma spp.to control plant soil-borne diseases is a promising field in biological control.In order to investigate the effect of T.asperellum T-6 on tobacco growth and resistance to soil-borne fungi,spore suspensions of T.asperellum T-6 were inoculated into soil,and then pot experiments were conducted in greenhouse.The results showed that hyphal growth of Phytophthora parasitica and Thielaviopsis basicola was inhibited by T.asperellum T-6,and the inhibited rates were 49.62%and 59.23%,respectively.Control efficiency of T.asperellum T-6 on black shank and black root rot reached 74.45%and 75.14%.After T.asperellum T-6 pretreatment,the content of chlorophyll and carotenoids and the activities of three resistance-related enzymes in tobacco leaves were increased,and the growth of tobacco treated with T.asperellum T-6 were better than control.Taken together,the results from this study indicated that T.asperellum T-6 could control tobacco black shank and black root rot effectively,and promote tobacco growth.

关 键 词：烟草 棘孢木霉T-6 黑胫病 根黑腐病 生物防治 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]