鸭油甘油二酯和维生素D3对结肠炎大鼠结肠菌群、脾脏过氧化物酶体增殖物激活受体γ mRNA表达量及结肠组织发育的影响  

Effects of Duck Oil Diglyceride and Vitamin D3 on Colonic Flora,Spleen Peroxisome Proliferators Activated Receptor γ mRNA Expression and Colonic Tissue Development of Colitis Rats

作　　者：刘亚楠 王宝维[1,2] 葛文华 张名爱 谢玉娥[1] 孔敏 孙京新 LIU Yanan;WANG Baowei;GE Wenhua;ZHANG Ming’ai;XIE Yu’e;KONG Min;SUN Jingxin(Department of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China;National Waterfowl Industry Technical System Nutrition and Feed Function Laboratory,Qingdao 266109,China)

机构地区：[1]青岛农业大学食品科学与工程学院,青岛266109 [2]国家水禽产业技术体系营养与饲料功能研究室,青岛266109

出　　处：《动物营养学报》2020年第3期1382-1395,共14页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家水禽产业技术体系专项基金(CARS-43-11);山东省2018年度农业重大应用技术创新项目

摘　　要：本试验旨在研究不同水平的鸭油甘油二酯和维生素D3对结肠炎大鼠结肠菌群、脾脏过氧化物酶体增殖物激活受体γ(PPARγ)mRNA表达量及结肠组织发育的影响。选择100只大鼠,随机分为模型组和对照组,对照组每组4个重复,模型组每组16个重复,每个重复均5只大鼠;对照组大鼠自由饮用蒸馏水,模型组大鼠自由饮用5%葡聚糖硫酸钠水溶液,进行连续7 d的造模试验。造模成功后,选取10只正常大鼠作为对照组(Ⅰ组),另选择建模成功的大鼠70只,随机分成7个组,分别为模型组(Ⅱ组)和试验组(Ⅲ~Ⅷ组);每组均2个重复,每个重复5只。采用2×3两因素交叉析因试验设计,鸭油甘油二酯添加水平分别为2.5、5.0、10.0 mL/kg,维生素D3添加水平分别为12.5、25.0μg/kg。试验期为3周。结果表明:1)鸭油甘油二酯与维生素D3交互作用对Ace指数、Chao1指数影响显著(P<0.05),与模型组相比,试验组操作分类单元(OTUs)数量、Ace指数、Chao1指数均显著增加(P<0.05),其中5.0 mL/kg鸭油甘油二酯和12.5μg/kg维生素D3组的OTUs数量、Ace指数、Chao1指数最高,微生物的物种丰度高于其他5个试验组。2)主成分分析图显示,5 mL/kg鸭油甘油二酯和12.5μg/kg维生素D3组与对照组距离最近,微生物相似性最高。3)与模型组相比,试验组的厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、γ-变形菌纲(Gammaproteobacteria)、志贺氏菌属(Shigella)、普拉氏菌属(Prevotella)和支原体菌属(Allobaculum)比例显著降低(P<0.05),拟杆菌门(Bacteroidetes)、拟杆菌属(Bacteroides)比例显著增加(P<0.05),梭状芽孢杆菌纲(Clostridia)比例无显著变化(P>0.05)。4)鸭油甘油二酯和维生素D3交互作用对绒毛高度、隐窝深度影响显著(P<0.05);与模型组相比,试验组的结肠绒毛高度、绒毛高度/隐窝深度显著增加(P<0.05),隐窝深度显著变浅(P<0.05)。5)鸭油甘油二酯和维生素D3交互作用对脾脏PPARγmRNA表达量影�This study aimed to explore the effects of different levels of duck oil diglyceride and vitamin D3 on colonic flora,spleen peroxisome proliferators activated receptorγ(PPARγ)mRNA expression and colonic tissue development of colitis rats.A total of 100 rats were randomly divided into model group and control group,each control group contained 4 replicates,each model group contained 16 replicates,and each replicate contained 5 rats;rats in control group free drank distilled water,rats in model group free drank 5%dextran sulfate sodium,and the modeling test for consecutive lasted for 7 days.After the successful molding,selected 10 normal rats as a control group(group Ⅰ),then selected 70 rats of successful molding randomly divided into 7 group,which were model group(group Ⅱ)and experimental groups(groups Ⅲ to Ⅷ);each group contained 2 replicates and each replicate contained 5 rats.A 2×3 cross factor analysis experiment design was adopted,the addition levels of duck oil diglyceride were 2.5,5.0 and 10.0 mL/kg,respectively,and the addition levels of vitamin D3 was 12.5 and 25.0μg/kg,respectively.The experiment lasted for 3 weeks.The results showed as follows:1)the interaction of duck oil diglyceride and vitamin D3 had significant effects on Ace index and Chao1 index(P<0.05),compared with the model group,the operation classification unit(OTUs)number,Ace index and Chao1 index of experimental groups were significantly increased(P<0.05),the OTUs number,Ace index and Chao1 index of 5.0 mL/kg duck oil diglyceride and 12.5μg/kg vitamin D3 group were the highest,and the microbial species abundance was higher than other five experimental groups.2)The principal component analysis figure showed that the 5.0 mL/kg duck oil diglyceride and 12.5μg/kg vitamin D3 group was closest to the control group,with the highest microbial similarity.3)Compared with the model group,the proportions of Firmicutes,Proteobacteria,Gammaproteobacteria,Shigella,Prevotella and Allobaculum of experimental groups were significantly decreased(P

关 键 词：鸭油甘油二酯 维生素D 3 结肠炎 大鼠 结肠菌群 肠道发育 PPARγ 

分 类 号：S816.7[农业科学—饲料科学]