基于相关性分析的金银花和山银花正丁醇提取物的抗肿瘤活性谱-效关系研究  

Spectra-effect Relationship of Antitumor Effects of n-Butanol of Lonicera japonicae Flos and Lonicera Flos Based on Correlation Analysis

作　　者：万丹[1,2,3] 张水寒 沈冰冰[1] 袁清照 蔡萍 WAN Dan;ZHANG Shuihan;SHEN Bingbing;YUAN Qingzhao;CAI Ping(Hunan Institute of TCM,Changsha 410013;Hunan TCM Raw Material Quality Monitoring Technology Service Center,Changsha 410013;2011 Collaboration and Innovation Center for Digital Chinese Medicine in Hunan,Changsha 410013)

机构地区：[1]湖南省中医药研究院,湖南长沙410013 [2]湖南省中药原料质量监测技术服务中心,湖南长沙410013 [3]湖南省2011数字中医药协同创新中心,湖南长沙410013

出　　处：《分析科学学报》2020年第1期37-41,共5页Journal of Analytical Science

基　　金：湖南省自然科学基金(No.2016JJ6086);湖南省中医药管理局项目(201702);湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开发基金(No.ZYFT201508)

摘　　要：本文采用相关性分析,研究了金银花和山银花正丁醇提取物的高效液相色谱-串联质谱(HPLC-MS/MS)图谱与抗肿瘤活性的谱-效关系。首先采用HPLC-MS/MS法对金银花和山银花正丁醇提取物进行分析,得到各提取物的化学成分,同时采用噻唑蓝(MTT)法观察金银花和山银花正丁醇提取物对Mcf-7、Hela、Ht29细胞株的抑制作用,然后利用最小二乘法分析其谱-效关系,通过分析化学成分与药效活性的相关性,从中筛选出具有代表性的化合物,进而探索金银花和山银花抗肿瘤作用的药效物质基础。The spectra-effect relationship between high performance liquid chromatography figerprints and antitumor effects of n-butanol extracts of Lonicera japonicae Flos and Lonicera Flos was studied.HPLC-MS/MS method was used to analyze n-butyl alcohol extracts from Lonicera japonicae Flos and Lonicera Flos,the inhibition of n-butyl alcohol extracts from Lonicera japonicae Flos and Lonicera Flos to Mcf-7,Hela and Ht29 was observed by MTT method,and the effect relationship was analyzed by least square method.In this study,the representative compounds were screened by analyzing the correlation between chemical constituents and pharmacodynamic activities,which provides a way to explore the pharmacodynamic material base of Lonicera japonicae Flos and Lonicera Flos.

关 键 词：金银花和山银花正丁醇提取物 抗肿瘤作用 谱-效关系 

分 类 号：O657.63[理学—分析化学]