芦荟大黄素在石墨烯/聚多巴胺/金复合纳米材料修饰电极上的电化学行为及检测  

Electrochemical Behaviors and Determination of Aloe Emodin at Graphene/Polydopamine/Au Nanocomposites Modified Electrode

作　　者：阳敬 兰慧 吴其国[2] 蓝伦礼 庄晨曦 赵佳 YANG Jing;LAN Hui;WU Qiguo;LAN Lunli;ZHUANG Chenxi;ZHAO Jia(Honghe Health Vocational College,Mengzi 661199;Anqing Medical College,Anqing 246052;Guilin Medical University,Guilin 541100)

机构地区：[1]红河卫生职业学院,云南蒙自661199 [2]安庆医药高等专科学校,安徽安庆246052 [3]桂林医学院,广西桂林541100

出　　处：《分析科学学报》2020年第1期95-100,共6页Journal of Analytical Science

基　　金：云南省教育厅资助性项目(No.2016ZZX296)

摘　　要：制备了石墨烯/聚多巴胺/金(GNs/PDA/Au)复合纳米材料,采用紫外-可见吸收光谱、X射线衍射及扫描电镜对合成的材料进行表征,并将该材料用于构建电化学传感器对芦荟大黄素进行检测。采用循环伏安法(CV)和差分脉冲伏安法(DPV)对影响芦荟大黄素检测的pH和富集时间进行优化,最佳条件为pH=5.72,富集时间280 s。在最佳实验条件下,GNs/PDA/Au修饰电极对芦荟大黄素检测的峰电流与芦荟大黄素浓度呈良好的线性关系,线性范围为16.03~125.8μmol/L,检测限为4.82μmol/L。可用于中药材中芦荟大黄素的定量测定。Determination of aloe rhubarb was conducted based on graphene/polydopamine/Au(GNs/PDA/Au)nanocomposites.The nanocomposites were characterized by UV-Vis spectroscopy,X ray diffraction and scan electron microscope.And then,the electrochemical sensor was fabricated using the prepared nanocomposites.The electrochemical behavior of aloe emodin was studied.The influence of pH and accumulation time was studied by cycle voltammetry and differential pulse voltammetry.Aloe emodin was detected under the optimal conditions,i.e.,pH of 5.72 and accumulation time of 280 s.Under this optimal condition,the linear response range for aloe emodin was 16.03-125.8μmol/L with the detection limit of 4.82μmol/L.The results indicated GNs/PDA/Au composite nanoparticles had a good linear relationship between the peak current and concentration of aloe emodin.The method might open up a new possibility for the widespread use of electrochemical sensors for monitoring of flavonoid drugs.

关 键 词：石墨烯 聚多巴胺 金纳米粒子 芦荟大黄素 电化学检测 

分 类 号：O657.1[理学—分析化学]