三波长吸收光谱法测定药物中地奥司明  

Determination of Diosmin in Drugs by Three-Wavelength Absorption Spectrometry

作　　者：张淑琼[1] 江虹[1] 刘艳[1] 向容 李琴 ZHANG Shuqiong;JIANG Hong;LIU Yan;XIANG Rong;LI Qin(College of Chemistry and Chemical Engineering,Collaborative Innovation Center for Green Development in Wuling Mountain Areas,Yangtz Normal University,Chongqing 408100)

机构地区：[1]长江师范学院化学化工学院长江师范学院武陵山片区绿色发展协同创新中心

出　　处：《分析科学学报》2020年第1期135-138,共4页Journal of Analytical Science

基　　金：重庆市教委科技基金(No.KJ1401226);长江师范学院科技基金(No.2018CXX117)

摘　　要：在弱酸性溶液中,地奥司明与亮绿反应生成具有3个强度相近负吸收峰的绿色二元离子缔合物。他们的负吸收波长分别位于424 nm、560 nm和660 nm处,表观摩尔吸光系数分别为5.84×10^4 L/(mol·cm)、6.02×10^4 L/(mol·cm)和5.76×10^4 L/(mol·cm)。当以双波长法(424 nm+560 nm)和三波长法(424 nm+560 nm+660 nm)测定时,其表观摩尔吸光系数分别达1.19×10^5 L/(mol·cm)和1.77×10^5 L/(mol·cm),线性范围均为0.03~12.2 mg/L。将灵敏度最高的三波长法用于市售地奥司明药物中地奥司明的测定,加标回收率为98.1%~102%,相对标准偏差(RSD,n=5)为2.0%~2.4%。In a weakly acidic solution,d iosmin reacted with brilliant green to form a green binary ion association complex with three negative absorption peaks similar in strength.Their negative absorption wavelengths were located at 424 nm,560 nm and 660 nm,respectively.The apparent molar absorptivities were 5.84×10^4 L/(mol·cm),6.02×10^4 L/(mol·cm)and 5.76×10^4 L/(mol·cm),respectively.When measured by double wavelength method(424 nm+560 nm)and three-wavelength method(424 nm+560 nm+660 nm),the apparent molar absorptivities could reach 1.19×10^5 L/(mol·cm)and 1.77×10^5 L/(mol·cm).The linear range was from 0.03 to 12.2 mg/L.The three-wavelength method with the highest sensitivity has been applied to the determination of diosmin in commercially available diosmin drugs,and the spiked recoveries and RSD(n=5)were in the ranges of 98.1%-102% and 2.0%-2.4%,respectively.

关 键 词：亮绿 地奥司明 三波长 吸收光谱法 

分 类 号：O657.32[理学—分析化学]