高效液相色谱手性流动相添加剂法拆分愈创甘油醚对映体  

Enantiomeric Separation of Guaifenesin by High Performance Liquid Chromatography with Chiral Mobile Phase Additives

作　　者：翟明翚[1] 韩爽[1] 王颖[1] 陈志伟 苏立强[1] ZHAI Minghui;HAN Shuang;WANG Ying;CHEN Zhiwei;SU Liqiang(College of Chemistry and Chemical Engineering,Qiqihar Uiniversity,Qiqihar 161006)

机构地区：[1]齐齐哈尔大学化学与化学工程学院

出　　处：《分析科学学报》2020年第1期139-142,共4页Journal of Analytical Science

基　　金：黑龙江省自然科学基金(No.QC2018073);黑龙江省教育厅项目(No.135109201,135209224)

摘　　要：以羧甲基-β-环糊精(CM-β-CD)作为手性流动相添加剂,采用高效液相色谱法(HPLC)拆分愈创甘油醚对映体。考察了添加剂的种类及浓度、有机修饰剂的种类及含量、流动相pH值、三乙胺体积浓度和流速对拆分的影响。优化的色谱分离条件为:C 18色谱柱,流动相为含0.5 g/L CM-β-CD的甲醇-乙腈-水(体积比为15∶70∶15),流速为0.2 mL/min,pH=4.26,三乙胺体积浓度为0.1%,紫外检测波长为226 nm。结果表明:在上述条件下,愈创甘油醚对映体的分离度R s为1.54。The enantiomeric separation of guaifenesin by high performance liquid chromatography(HPLC)was studied using carboxymethyl-β-cyclodextrin(CM-β-CD)as chiral mobile phase additive.The effects of types and content of chiral mobile phase additive and organic modifier,the pH value,the volume concentration of triethylamine,and the flow rate on the enantiomer separation were investigated.The best separation was obtained when C 18 column was used and the mobile phase consisted of 0.1%(V/V)triethylamine and methanol-acetonitrile-water(15:70:15,V/V/V)containing 0.5 g/L CM-β-CD with pH value of 4.26 at the flow rate of 0.2 mL/min.The detection wavelength was set at 226 nm.Under the optimized conditions,the resolution of enantiomers was 1.54.

关 键 词：高效液相色谱 手性流动相添加剂 拆分 愈创甘油醚对映体 

分 类 号：O657.72[理学—分析化学]