等钳蠊螨联苯肼酯抗敏品系对柑橘全爪螨的捕食作用  

Predatory function of bifenazate-resistant strain of Blattisocius dentriticus Berlese

作　　者：周浩楠 潘琦[1] 侯栋元 杨娟生 于士将[1] 成禄艳 雷双 王莉 丛林[1] 冉春[1] ZHOU Haonan;PAN Qi;HOU Dongyuan;YANG Juansheng;YU Shijiang;CHENG Luyan;LEI Shuang;WANG Li;CONG Lin;RAN Chun(Citrus Research Institute,Southwest University,Chongqing 400712,China)

机构地区：[1]西南大学柑桔研究所

出　　处：《果树学报》2020年第2期264-271,共8页Journal of Fruit Science

基　　金：国家重点研发计划（2018YFD0201508）;中国农业科学院科技创新工程协同创新任务（CAAS-XTCX-2016013）;重庆市科技创新领军人才支持计划（cstc2018kjcxljrc0040）;西南大学博士基金（SWU116061）

摘　　要：【目的】探究不同温度下等钳蠊螨(Blattisocius dentriticus Berlese)联苯肼酯抗性品系和敏感品系对柑橘全爪螨(Panonychus citri McGregor)的捕食能力差异。【方法】采用室内生物测定的方法,研究了在16、20、24、28、32℃条件下等钳蠊螨联苯肼酯抗性品系和敏感品系对柑橘全爪螨卵和雌成螨的捕食作用。【结果】等钳蠊螨联苯肼酯抗性品系和敏感品系对柑橘全爪螨卵和雌成螨的捕食量没有显著差异。【结论】联苯肼酯抗性获得并未影响等钳蠊螨对柑橘全爪螨的捕食功能,因此,等钳蠊螨有发展成为生防天敌的潜力。【Objective】The objective of this research was to clarify the predatory functional response of the bifenazate-resistant strain of Blattisocius dentriticus to Panonychus citri and evaluate whether it was possible to use the"alternative prey method"to raise B. dentriticus and whether it had the potential to become a natural enemy of citrus, which aimed to better coordinate the use of predatory mites and miticides, and to guide the effective combination of field chemical and biological control.【Methods】Resistance was evaluated using biological assays conducted in a laboratory. The predatory effect of the equal clamp on the elliptic meal was determined by the method of Mcmurtry and Scriven. Round citrus leaves with a diameter of 0.70 cm were placed in each groove of a six-concave slide with a diameter of 1.50 cm and a depth of 0.22 cm, respectively. Aleuroglyphus ovatus at the density of 3, 6, 9, 12, 15, and 18 heads was placed in each of the grooves, and the B. dentriticus was placed into a drop of sputum, each of which was placed in a tongs(hungry after 24 h). It was covered with a cover slip to prevent cockroaches from escaping, and covered with a wet brush. A circle of water film was applied around the film as isolation(4 h once), and the samples were put into the artificial climate chamber. Five temperature treatments was set separately and the predation effects were observed at 16, 20, 24, 28, 32 ℃ after 24 hours. The relative humidity was(80 ± 5)%, the photoperiod was 14 L: 10 D, and each treatment was repeated for three times. The predatory functional response model of the B. dentriticus was fitted to the Holling disc equation,i.e., N_a=αTN/(1 + αT_hN). Where N_awas the number of prey;α was the instantaneous attack rate;T was the total test time;N was the prey density, which was the initial access to the prey density;and Thwas the time taken by the predator to process each prey. The test time was one day.The α/Thvalue was used to evaluate the predation ability of the equal clamp.【Results】Hol

关 键 词：等钳蠊螨 柑橘全爪螨 联苯肼酯 捕食功能反应 生物防治 

分 类 号：S666[农业科学—果树学]