水库沉积物贫营养型好氧反硝化菌(Pseudomonas sp.)分离及其对微污染水体脱氮效率分析  

Isolation of aerobic denitrifying bacteria(Pseudomonas sp.) from reservoir sediments and analysis of its denitrification efficiency in micro-polluted water

作　　者：王慎 张思思 许尤 官卓宇 杨正健[2] 刘德富 马骏[1] WANG Shen;ZHANG Sisi;XU You;GUAN Zhuoyu;YANG Zhengjian;LIU Defu;MA Jun(Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization,School of Civil Engineering,Architecture and Environment,Hubei University of Technology,Wuhan 430068;Engineering Research Center of Ecoenvironment in Three Gorges Reservoir Region,Ministry of Education,Three Gorges University,Yichang 443002)

机构地区：[1]湖北工业大学土木建筑与环境学院,河湖生态修复与藻类利用湖北省重点实验室,武汉430068 [2]三峡大学,三峡库区生态环境教育部工程研究中心,宜昌443002

出　　处：《环境科学学报》2020年第3期915-921,共7页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金项目(No.91647207,51879099,51779128)

摘　　要：为探究深水水库沉积物微生物功能特征及利用价值,于2019年在实验室对小湾水库表层沉积物微生物进行了驯化分离,并分析了其中一株细菌的脱氮效率.结果表明,分离出的细菌XW731经鉴定属于假单胞菌属(Pseudomonas sp.),是一种贫营养型好氧反硝化菌;在分别以NH4^+-N、NO3^--N和NO2^--N为唯一氮源时,该菌对NH4^+-N、NO3^--N和NO2^--N去除率分别为33.6%、68.5%和9.1%;以NH4^+-N和NO3^--N为氮源时,对NH4^+-N和NO3^--N去除率分别为66.4%、89.6%,同步硝化反硝化能力更强.将该菌投加到两种城市微污染水体后测试表明,该菌对城市河道水体的NH4^+-N和NO3^--N去除率分别为38.3%和42.4%,对城市降雨水体的NH4^+-N和NO3^--N去除率分别为22.2%和7.7%.In order to study the characteristics and application of microorganisms in sediments of deep-water reservoir,the microorganisms in surface sediments of the Xiaowan reservoir were cultivated and isolated in the laboratory,and the denitrification efficiency of one isolate was analyzed.The results show that the isolate,XW731,is a type of oligotrophic aerobic denitrifying bacteria,which belongs to Pseudomonas.When NH4^+-N,NO3^--N and NO2^--N was the sole nitrogen source,respectively,the removal rates of NH4^+-N,NO3^--N and NO-2-N were 33.6%,68.5% and 9.1%.when NH4^+-N,NO3^--N were nitrogen sources,the removal rates of NH4^+-N,NO3^--N were 66.4%,89.6%,meanwhile,simultaneous nitrification and denitrification ability were stronger.Furthermore,the bacteria were added to two kinds of micro-polluted water,XW731 had certain denitrification effect and the removal rates of NH4^+-N,NO3^--N were 38.3%,42.4% respectively in urban river water,and 22.2%,7.7%respectively in urban rainfall water.

关 键 词：好氧反硝化菌 异养硝化 脱氮特性 

分 类 号：X522[环境科学与工程—环境工程]