应用SS-PCR技术建立番石榴实蝇快速鉴定方法  

Rapid Identification of Bactrocera correcta (Bezzi) by SS-PCR Technique

作　　者：黄振[1,2] 侯有明 郭琼霞[3] HUANG Zhen;HOU You-ming∗;GUO Qiong-xia(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fuzhou Changle Airport Customs,Fuzhou 350209,China;Fuzhou Customs Inspection and Quarantine Technical Center,Fuzhou 350001,China)

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]福州长乐机场海关,福建福州350209 [3]福州海关检验检疫技术中心,福建福州350001

出　　处：《江西农业学报》2020年第2期66-70,共5页Acta Agriculturae Jiangxi

基　　金：福建省自然科学基金项目(2011J01066、2012JO1061)

摘　　要：应用种特异引物PCR(Species-specific PCR, SS-PCR)技术,基于mt DNA COⅠ线粒体基因系列,设计了1对能够准确鉴定番石榴实蝇Bactrocera(Bactrocera)correcta(Bezzi)的种特异性引物FR447和FF463-486,选用番石榴实蝇作为阳性对照,以杨桃实蝇B.carambolae Drew&Hancock等其他19种实蝇作为阴性对照,进行PCR扩增并将PCR产物进行电泳检测。结果表明:仅番石榴实蝇能够在约645 bp位置扩增出1条清晰且单一的目的条带。将本实验建立的SS-PCR鉴定方法应用在实际检疫工作中,得到了验证。Based on Species-specific PCR(SS-PCR) technique and the mt DNA COⅠ, a pair of species-specific primers FR447 and FF463-486, which could accurately identify Bactrocera correcta(Bezzi), were designed for PCR amplification and gel electrophoresis. B. correcta was used as the positive control, and the other 19 species of fruit flies including B. carambolae Drew & Hancock were used as the negative control. The results showed that Only B. correcta can be amplified 645 bp band, which was clear and single. The other fruit flies were lack of band. The application of SS-PCR identification method which established in this experiment has been verified in real work.

关 键 词：番石榴实蝇 种特异性引物 SS-PCR mt DNA COⅠ 快速鉴定 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]