非洲栽培稻垩白粒率耐热性QTL的定位  

QTL Mapping for Heat Tolerance of Chalky Grain Rate of Oryza glaberrima Steud.

作　　者：曹志斌 李瑶[2] 曾博虹 毛凌华 蔡耀辉 吴晓峰 袁林峰 CAO Zhibin;LI Yao;ZENG Bohong;MAO Linghua;CAI Yaohui;WU Xiaofeng;YUAN Linfeng(Laboratory of Rice National Engineering,Jiangxi Research and Development Center of Super Rice,Nanchang 330200,China;Institute of Soil and Fertilizer&Resources and Environment,Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River,Ministry of Agriculture,Nanchang 330200,China)

机构地区：[1]江西省超级水稻研究发展中心/国家水稻工程实验室(南昌),南昌330200 [2]江西省农业科学院土壤肥料与资源环境研究所/农业部长江中下游作物生理生态与耕作重点实验室,南昌330200

出　　处：《中国水稻科学》2020年第2期135-142,共8页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(31560382);江西省杰出青年科学基金资助项目(2018ACB21026);江西省自然科学基金资助项目(20151BAB214013; 2017BAB204020);江西省现代农业科研协同创新专项(JXXTCXFY201902);国家重点研发计划资助项目(2017YFD0301601; 2018YFD0301103);江西省重点研发计划资助项目(20171ACF60019)

摘　　要：【目的】本研究旨在定位一个稻米垩白粒率高温耐性QTL,为外观品质育种及解析垩白粒率高温耐性的遗传机制提供依据。【方法】以非洲栽培稻耐热品种IRGC102309(Oryza glaberrima Steud.)和籼稻品种R9311(O. sativa L. subsp. indica Kato.)为亲本构建的栽培稻种间染色体片段导入系CSIL05-23为材料构建次级分离群体,结合人工气候室模拟灌浆期高温胁迫处理,采用垩白粒率高温钝感值为评价指标,对非洲栽培稻垩白粒率高温耐性QTL进行检测。【结果】在BC6F2分离群体,利用单标记分析,发现第5染色体上的SSR标记RM1200与垩白粒率耐热性状极显著正相关(P=0.0005)。进一步利用BC6F3和BC6F4分离群体,采用QTL Cartographer 2.5软件和复合区间作图法在水稻第5染色体上的SSR标记RM1200-RM5796区间重复检测到一个灌浆期垩白粒率耐热性QTL,命名为qHTCGR5,分别解释11.4%和17.5%表型变异。根据BC6F4分离群体的纯合重组体表型分组,利用置换作图方法将目标QTL同样定位在SSR标记RM1200-RM5796之间,遗传图距为1.3 cM,物理图距约为333.4 kb。【结论】控制垩白粒率耐热性的qHTCGR5是一个能够用于稻米外观品质育种的新QTL。【Objective】The objective of the research is to identify a QTL for heat tolerance of chalky grain rate, so as to provide support for the appearance quality breeding and analysis of genetic mechanism of rice high temperature tolerance of chalky grain rate. 【Method】 We developed a chalky grain rate heat-tolerant CSIL(chromosomal segment introgression lines), CSIL05-2, by backcrossing and marker assisted selection with African cultivated rice acc. IRGC102309(Oryza glaberrima Steud.) as donor parent and Asian cultivated rice R9311(O. sativa L. subsp. indica Kato) as recipient parent. And a QTL for insensitity of heat tolerance of chalky grain rate on chromosome 5 was analyzed using secondary populations from CSIL05-2.【Results】In a BC6 F2 segregation populations, the SSR marker RM1200 on chromosome 5 showed significant correlation with heat tolerance of chalky grain rate by single marker analysis(P=0.0005). Using Cartographer 2.5 and the composite interval mapping with BC6 F3 and BC6 F4 populations, we further anchored the quantitative trait loci(QTL) associated with heat tolerance of chalky grain rate at filling stage to the same position within a 1.3 c M interval, which was designed as qHTCGR and explained 11.4% and 17.5% of the phenotypic variances, respectively. Using homozygous recombinants screened from BC6 F4 populations, qHTCGR5 was also detected in a ~333.4 kb region between RM11633 and RM11642.【Conclusion】 The QTL controlling the heat tolerance of chalky grain rate qHTCGR5 is a novel QTL.

关 键 词：水稻 垩白粒率 耐热性 数量性状位点 基因定位 

分 类 号：Q343.1[生物学—遗传学] S511.32[农业科学—作物学]