几种生防细菌对尖孢镰刀菌的抑菌效果及其相互间包容性生长的快速检测  

Inhibitory Effect of Some Biocontrol Bacteria on Fusarium oxysporum and Rapid Detection of the Biocontrol Bacteria for Their Inclusive Growth

作　　者：孙茜茜 李春强[1] 易小平[1] SUN Qianqian;LI Chunqiang;YI Xiaoping(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101)

机构地区：[1]中国热带农业科学院热带生物技术研究所/农业农村部热带作物生物学与遗传资源利用重点实验室

出　　处：《热带农业科学》2019年第12期36-41,共6页Chinese Journal of Tropical Agriculture

基　　金：海南省重点研发计划(No.ZDYF2018036);中国热带农业科学院基本科研业务费(No.1630052018003,No.1630052019028,No.1630052016005)

摘　　要：为明确几种生防细菌对茄子枯萎病菌和香蕉枯萎病菌的抑菌效果,采用平板对峙法进行抑菌试验。结果表明,JK2015菌对茄子枯萎病菌和香蕉枯萎病菌的抑菌效果最为明显,抑菌率分别为52.54%和70.45%;而巨大芽孢杆菌和地衣芽孢杆菌对2种病原真菌均无明显的抑菌效果,抑菌率只有3.48%~7.25%;对2种病原菌的抑菌效果均为:JK2015菌>胶冻样芽孢杆菌>JK02菌>JK01菌>枯草芽孢杆菌>解淀粉芽孢杆菌。同时,为了明确生防菌之间的生长是否相互影响,采用平板划线共培养,探索出一种快速检测方法,对它们之间包容性做出快速初筛,结果表明,JK01和JK02不能与枯草芽孢杆菌、解淀粉芽孢杆菌包容生长,枯草芽孢杆菌不能与胶冻样芽孢杆菌包容生长,而其余菌株间都可以包容生长。这些结果可为微生物混合菌剂及茄子和香蕉专用生物肥料的开发奠定基础。In order to clarify inhibitory effect of some biocontrol bacteria on pathogens Fusarium oxysporum f.sp.melongenae and Fusarium oxysporum f.sp.cubense,the plate dual-culture antagonistic method was used to carry out an inhibition spectrum test.The results showed that bacterial strain JK2015 had the most obvious inhibitory effect on F.oxysporum f.sp.Melongenae and F.oxysporum f.sp.cubense,with the inhibition rates being 52.54%and 70.45%,respectively.However,Bacillus megaterium and Bacillus licheniformis had no obvious inhibitory effect on the two pathogenic fungi,and their inhibition rates were only ranged from 3.48%to 7.25%.The inhibitory effects of the biocontrol bacteria on the two pathogens were in the order of JK2015>Bacillus licheniformis>JK02>JK01>Bacillus subtilis>Bacillus amyloliquefaciens.At the same time in order to clarify whether these biocontrol bacteria could had an inclusive growth,a fast test medium was developed.The inclusive growth test of the biocontrol bacteria was conducted by scribing co-culture.The results showed that most of the bacterial strains grew inclusively,except JK01 and JK02 which failed to grow inclusively with Bacillus subtilis and Bacillus amyloliquefaciens,and except Bacillus subtilis failing to grow inclusively with Bacillus licheniformis.These results laid a foundation for the development of mixed microbial agents and special bio-fertilizers for eggplant or banana.

关 键 词：生防菌 茄子枯萎病菌 香蕉枯萎病菌 尖孢镰刀菌枯萎病 包容性生长 

分 类 号：S482.2[农业科学—农药学]