浅析透射电子显微镜在生物学科中的应用  

Current Application of Transmission Electron Microscope in Biology

作　　者：李叶[1,2] 黄华平[1,2] 张新春[1,2] 崔艳梅 邓睿[1,2] 林培群 LI Ye;HUANG Huaping;ZHANG Xinchun;CUI Yanmei;DENG Rui;LIN Peiqun(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101;Key Laboratory of Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests,Danzhou,Hainan 571737)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101 [2]农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737

出　　处：《热带农业科学》2019年第12期58-67,共10页Chinese Journal of Tropical Agriculture

基　　金：中央级公益性科研院所基本科研业务费国际合作专项(No.1630042019012);中央级公益性科研院所基本科研业务创新人才培养和团队建设专项(No.10630042019039)

摘　　要：透射电镜具有高分辨率、直观性的特点,是研究微观组织结构的强有力工具,在材料、医学、生物、化学、物理等各个研究领域发挥着不可替代的重要作用。本文结合生物样品的特点,介绍了透射电子显微镜的成像原理、生物样品的制备,综述了透射电子显微镜作为生物组织、细胞及细胞器超微结构观察工具在生物学科中的应用,为透射电镜的初学者以及科研工作人员全面了解和使用透射电镜提供参考。Transmission electron microscopy(TEM)is a powerful instrument for microstructural study since it has a high resolution and intuitiveness.It is widely used in various fields such as material,medicine,biology,chemistry,physics,etc.The features of biological samples,the imaging principle and preparation of biological samples in TEM were introduced,and the application of TEM as an observation tool for biological tissues,cells and organelle ultrastructures were reviewed.This might provide some references to the beginner and scientists for full understanding and application of TEM.

关 键 词：透射电镜 成像原理 生物学 应用 

分 类 号：TN16[电子电信—物理电子学]